Summary
Synaptosomes isolated from post mortem human brain release the transmitter amino acids upon depolarisation. Active preparations can be isolated for up to 24 hours post mortem. The amount of depolarisationinduced release of the transmitter amino acids is correlated with the rate of oxygen uptake. Only when this correlation is taken into consideration would it be possible to detect disease-related changes in presynaptic aminoacid transport systems.
Similar content being viewed by others
References
Bateman, D. E., Hardy, J. A., Dodd, P. R., Kidd, A. M., Perry, R. H., Edwardson, J. A., Singh, G. B.: The effect of the agonal state on the metabolic activity of nerve endings isolated from post mortem human brain. J. Neurol. Sci. (submitted, 1984).
Cheetham, S. C., Dodd, P. R., Edwardson, J. A., Hardy, J. A., Kidd, A. M.: Kinetics of GABA uptake into synaptosomes from frozen, post mortem human brain. Br. J. Pharm.79, 267 P (1983).
Cunningham, J., Neal, M. J.: On the mechanism by which veratrine causes a calcium-independent release ofγ aminobutyric acid from brain slices. Br. J. Pharm.73, 655–667 (1981).
Dodd, P. R., Hardy, J. A., Bradford, H. F., Edwardson, J. A., Harding, B. N.: Metabolic and Secretory processes in nerve-endings isolated from post mortem brain. Neurosci. Letts.11, 87–92 (1979).
Dodd, P. R., Hardy, J. A., Oakley, A. E., Strong, A. J.: Synaptosomes prepared from fresh human cerebral cortex: morphology respiration and release of transmitter amino acids. Brain Res.224, 419–425 (1981a).
Dodd, P. R., Hardy, J. A., Oakley, A. E., Edwardson, J. A., Perry, E. K., Delaunoy, J.-P.: A rapid method for preparing synaptosomes: comparison with alternative procedures. Brain Res.226, 107–118 (1981b).
Gray, E. G., Whittaker, V. P.: The isolation of nerve endings from brain: an electronmicroscopic study of cell fragments divided by homogenization and centrifugation. J. Anat.96, 79–88 (1962).
Hardy, J. A., Dodd, P. R.: Metabolic and functional studies on post mortem human brain. Neurochem. Internat.5, 253–266 (1983).
Hardy, J. A., De Belleroche, J. S., Border, D., Bradford, H. F.: Differential transmitter release from nerve terminals isolated from basal ganglia and substantia nigra. J. Neurochem.34, 1130–1139 (1980).
Hardy, J. A., Dodd, P. R., Oakley, A. E., Kidd, A. M., Perry, R. H., Edwardson, J. A.: Use of post mortem human synaptosomes for studies of metabolism and transmitter amino acid release. Neurosci. Letts.33, 317–322 (1982).
Hardy, J. A., Dodd, P. R., Oakley, A. E., Perry, R. H., Edwardson, J. A., Kidd, A. M.: Metabolically active synaptosomes can be prepared from frozen rat und human brain. J. Neurochem.40, 608–614 (1983).
Lowry, O. H., Rosenbrough, N. S., Farr, A. C., Randall, R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem.93, 265–275 (1951).
Schwarcz, R., Whetsell, W. O.: Post-mortem high affinity glutamate uptake in human brain. Neurosci.7, 1771–1778 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hardy, J.A., Bateman, D.E., Kidd, A.M. et al. Amino acid transport by synaptosomes isolated from post mortem human brain. J. Neural Transmission 60, 57–62 (1984). https://doi.org/10.1007/BF01254765
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01254765