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Zytogenetische Charakterisierung der Zellinien BHK-21 C13 und BHK-21 C13 S

Cytogenetic studies of the cell lines BHK-21 C 13 and BHK-21 C 13 S

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Zusammenfassung

221 Metaphasen der Zellinie BHK-21 C13 in der 81. Passage und 227 Metaphasen der Linie BHK-21 C13S in der 80. Passage wurden ausgewertet. BHK-21 C13 besitzt eine Stammlinie (65%) mit 44 Chromosomen. Die Karyotypierung von 10 Mitosen der Stammlinie ergab keine Abweichungen vom diploiden Komplement des männlichen Syrischen Hamsters (Mesocricetus auratus). Die aneuploiden Metaphasen verteilen sich vorwiegend auf den hypodiploiden und hyperdiploiden Bereich. 4,8% der Mitosen haben eine annähernd tetraploide Chromosomenzahl. Bei der Linie BHK-21 C13S liegen zwei Stammlinien mit 42 (32,3%) bzw. 43 (24,2%) Chromosomen vor. Nur 9,8% der Mitosen weisen die diploide Chromosomenzahl 44 auf. Im Gegensatz zu der Linie BHK-21 C13 wurden hier selbst hypertetraploide Metaphasen und eine annähernd oktoploide Mitose gefunden. Die Karyotypen der Stammlinien stimmen in keinem der 12 untersuchten Fälle überein. Als gerichtete Veränderung gegenüber dem diploiden Karyotyp fällt bei 10 der 19 analysierten Mitosen mit 40–44 Chromosomen eine Trisomie des Chromosoms 21 auf. Bei 16,3% der Metaphasen konnten dizentrische, in drei Fällen trizentrische Chromosomen festgestellt werden. Die Befunde werden mit denen früherer Untersuchungen verglichen und diskutiert.

Summary

221 metaphases of the cell line BHK-21 C13 and 227 metaphases of the line BHK-21 C13S were examined. BHK-21 C13 has a stemline of 44 chromosomes (65%). The karyotypes of 10 mitoses of the stemline showed no dissimilarities as compared to the diploid complement of the male Syrian hamster (Mesocricetus auratus). Aneuploid metaphases were mainly of the hypodiploid and hyperdiploid range. 4.8% of the mitoses were near-tetraploid. The cell line BHK-21 C13S possessed two stemlines with 42 (23.3%) and 43 (24.2%) chromosomes. Only 9.8% of the mitoses examined had the diploid number 44 of chromosomes. Unlike the line BHK-21 C13, even hypertetraploid and one near-oktoploid metaphases were found. The karyotypes of the stemlines were heterogeneous in all 12 cases analysed. As a remarkable non-random deviation from the diploid complement a trisomic chromosome 21 was noted in 10 of 19 karyograms with 40–44 chromosomes. In 16.3% of the metaphases there were dicentric, in three cases tricentric chromosomes. The results are compared with those of former investigations and discussed.

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Author notes

  1. Anna B. Fischer & E. Kuwert

    Present address: Abteilung für Virologie und Immunologie des Instituts für Medizinische Mikrobiologie, Klinikum Essen der Ruhr-Universität, Deutschland

  2. N. Manojlovic & E. -G. Beck

    Present address: Medizinisches Institut für Lufthygiene und Silikoseforschung an der Universität Düsseldorf, Deutschland

Authors and Affiliations

  1. Abteilung für Virologie und Immunologie des Instituts für Medizinische Mikrobiologie, Klinikum Essen der Ruhr-Universität, Deutschland

    N. Manojlovic & E. -G. Beck

  2. Medizinisches Institut für Lufthygiene und Silikoseforschung an der Universität Düsseldorf, Deutschland

    Anna B. Fischer & E. Kuwert

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  1. Anna B. Fischer
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  2. N. Manojlovic
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Fischer, A.B., Manojlovic, N., Kuwert, E. et al. Zytogenetische Charakterisierung der Zellinien BHK-21 C13 und BHK-21 C13 S. Archiv f Virusforschung 33, 375–384 (1971). https://doi.org/10.1007/BF01254694

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  • DOI: https://doi.org/10.1007/BF01254694

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