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Use of B-IV zonal rotor centrifugation as a simple tool for the separation of adeno-associated X7 virus (AAV X7) from helper adenoviruses

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Summary

The use of zonal rotor centrifugation in the separation of AAV X7 virus from its helper adenovirus is reported. AAV X7 virus was perfectly separated from its helper adenovirus. Adeno-associated X7 virus was found to separate in par tides with an “S” rate of 72–82 “S”, and a density in CsCl of 1.30–1.32, and virions with a sedimentation coefficient of 135–147 Svedbergs, with a density of 1.37–1.38.

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References

  1. Anderson, N. G.: Virus isolation in the zonal ultracentrifuge. Nature (Lond.)199, 1166–1168 (1963).

    Google Scholar 

  2. Anderson, N. G., H. P. Barringer, E. F. Babelay, C. E. Nunley, M. J. Bartkus, W. D. Fisher, andC. T. Rankin: The design and operation of the B-IV zonal centrifuge system. Nat. Cancer Inst. Monogr.21, 137–164 (1966).

    PubMed  Google Scholar 

  3. Atchison, R. W., B. C. Casto, andW. McD. Hammon: Adenovirus-associated defective virus particles. Science149, 754–756 (1965).

    PubMed  Google Scholar 

  4. Fox, S. M., G. D. Birnie, D. R. Harvey, E. M. Martin, andJ. A. Sonnabend: The use of batch-type zonal ultracentrifuge rotors for the isolation and purification of viruses. J. gen. Virol.2, 455–459 (1968).

    PubMed  Google Scholar 

  5. Gerin, J. L., W. R. Richter, J. D. Fenters, andJ. C. Holper: Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses. J. Virol.2, 937–943 (1968).

    PubMed  Google Scholar 

  6. Hoggan, M. D., N. R. Blacklow, andW. P. Rowe: Studies of small DNA viruses found in various adenovirus preparations: physical, biological and immunological characteristics. Proc. nat. Acad. Sci. (Wash.)55, 1467–1474 (1966).

    Google Scholar 

  7. Ito, M., andH. D. Mayor: Hemagglutinin of type 4 adeno-associated satellite virus. J. Immunol.100, 61–68 (1968).

    PubMed  Google Scholar 

  8. Luchsinger, E., R. Strobbe, G. Wellemans, D. Dekegel, andS. Sprecher-Goldberger: Haemagglutinating adeno-associated virus (AAV) in association with bovine adenovirus type 1. Arch. ges. Virusforsch.31, 390–392 (1970).

    PubMed  Google Scholar 

  9. Leunen, J., M. Mammerickx, etR. Strobbe: La préparation industrielle des cultures cellulaires à l'Institut national de recherches vétérinaires de Bruxelles, par la technique des flacons roulants. Bull. Off. int. Epiz.59, 1019–1036 (1963).

    Google Scholar 

  10. Mayor, H. D., R. M. Jamison, L. E. Jordan, andJ. L. Melnick: Structure and composition of a small particle prepared from a simian adenovirus. J. Bact.90, 235–242 (1965).

    Google Scholar 

  11. Mayor, H. D., K. Torikai, J. L. Melnick, andM. Mandel: Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions. Science166, 1280–1282 (1969).

    PubMed  Google Scholar 

  12. McEwen, C. R.: Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Analyt. Biochem.20, 114–149 (1967).

    PubMed  Google Scholar 

  13. Trautman, R.: Ultracentrifugation. In: Instrumental Methods of experimental Biology. 211–297, (D. W. Newmann ed.). New York: The Macmillan Co., 1964.

    Google Scholar 

  14. Neurath, A. R., J. T. Stasny, B. A. Rubin, R. W. Hartzell, andF. P. Wiener: Gel filtration on agarose in the separation of adeno-associated viruses from adeno-14 viruses and their structural components. J. gen. Virol.5, 451–454 (1969).

    Google Scholar 

  15. Takatst, G.: The use of spiral loops in serological and virological micromethods. Acta microbiol. Acad. Sci. hung.3, 191–202 (1955).

    PubMed  Google Scholar 

  16. Toplik, I., R. Boyden, A. De Padova, P. Brandt, andP. Sottong: The zonal centrifuge applied to the purification of a low-yield virus in human leukemia cells. Biotechn. Bioengin.10, 651–668 (1968).

    Google Scholar 

  17. Wellemans, G.: Examen sérologique de quelques troupeaux de bovins atteints de troubles respiratoires. Ann. Méd.-vét.1, 47–60 (1969).

    Google Scholar 

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Author notes

  1. R. Strobbe

    Present address: Institut National de Recherches Vétérinaires, Belgium

Authors and Affiliations

  1. Instituto de Microbiologia, Universidad Austral de Chile. Valdivia Scolarship, Ministère de l'Education National et de la Culture, Brussels, Belgium

    E. Luchsinger

  2. Laboratory for electron microscopy, Pasteur Institute, Brussels, Belgium

    D. Dekegel

  3. Institut National de Recherches Vétérinaires, Belgium

    E. Luchsinger, D. Dekegel & G. Wellemans

Authors
  1. E. Luchsinger
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  2. R. Strobbe
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  3. D. Dekegel
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  4. G. Wellemans
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Luchsinger, E., Strobbe, R., Dekegel, D. et al. Use of B-IV zonal rotor centrifugation as a simple tool for the separation of adeno-associated X7 virus (AAV X7) from helper adenoviruses. Archiv f Virusforschung 33, 251–258 (1971). https://doi.org/10.1007/BF01254681

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  • DOI: https://doi.org/10.1007/BF01254681

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