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Diethylaminoethyl-dextran requirement for poly I: C-indueed interferon effective against foot-and-mouth disease virus in vitro

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Summary

Interferon effective against foot-and-mouth disease virus was induced in bovine kidney cells by polyribonucleic acid (Poly I∶C) only when reacted with DEAE dextran. Furthermore, a high DEAE dextran to Poly I∶C ratio was necessary to elicit maximal response. Interferon was observed first in the cell and reached a peak at 8 hours after induction. Release of interferon from the cell reached significant levels after 8 hours and continued to accumulate in the supernatant fluid. Differences were observed between different preparations of Poly I∶C., which might be related to the extent of double-strandedness.

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Author notes

  1. J. Y. Richmond

    Present address: Plum Island Animal Disease Laboratory, Veterinary Sciences Research Division, Agricultural Research Service, U. S. Department of Agriculture, Greenport, Long Island, New York, USA

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    1. J. Y. Richmond
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    Part of this work was done while in pursuance of a Postdoctoral Resident Research Associateship of the Agricultural Research Service and the National Academy of Sciences, National Research Council.

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    Richmond, J.Y. Diethylaminoethyl-dextran requirement for poly I: C-indueed interferon effective against foot-and-mouth disease virus in vitro. Archiv f Virusforschung 33, 242–250 (1971). https://doi.org/10.1007/BF01254680

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    • DOI: https://doi.org/10.1007/BF01254680

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