Summary
Influenza A2/Singapore and A2/Hong Kong virus strains formed plaques in monolayers of monkey kidney and dog kidney cells. Using a serum-free overlay containing Earle's balanced salt solution with 1% lactalbumin hydrolysate, 1.4 g/1 sodium bicarbonate and 0.6% agarose, plaque formation occurred in 4–7 days. By incorporating compound in the overlay, plaque inhibition was demonstrated by Amantadine, Rimantadine and the isoquinoline derivatives designated Pfizer UK-2054, UK-2617, UK-2762 and UK-2888.
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Bentley, J., Wickham, E.A. A simple plaque assay for influenza A2 viruses and its application to antiviral screening. Archiv f Virusforschung 33, 234–241 (1971). https://doi.org/10.1007/BF01254679
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DOI: https://doi.org/10.1007/BF01254679