Summary
Aqueous extracts of sheep pineal bodies were separated on Sephadex G-25. A low molecular Sephadex G-25 fraction F 4 was filtrated on Sephadex G-10. Several distinct peaks could be detected showing excitation and fluorescence maxima resembling those of indoles.
A thinlayer chromatography study revealed that one Sephadex G-10 peak contained a spot identical with that of synthetic 5-methoxytryptophol, another a spot with the same Rf-values in different solvents as synthetic 5-hydroxytryptophol, while from a third peak a substance was isolated identical with synthetic melatonin. Mass spectra of the isolated compounds were recorded.
These results are compared with those of previous isolation studies.
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Ebels, I., Horwitz-Bresser, A.E.M. Separation of pineal extracts by gelfiltration. J. Neural Transmission 38, 31–41 (1976). https://doi.org/10.1007/BF01254138
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DOI: https://doi.org/10.1007/BF01254138