Summary
The inhibition titer of immune sera against vaccinia hemagglutination is inversely proportional to the concentration of hemagglutinin. Using the same number of hemagglutinin units, the sample of blood or hemagglutinin and the temperature of the reaction is irrelevant. Inaetivation of the sera at 56° to 62° does not influence the reaction. The serum titers are higher, when more diluted blood cell suspensions are used, as well as vice versa. A prolonged contact between serum and hemagglutinin shows little influence on the titer. The inhibition is stronger at the acid than at the alcaline range (pH 6 to 9). The reproducibility of the method is checked. Normal animal sera do not show any non-specific inhibition.
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Ich danke den HerrenGiese, Reinsdorf undSander für ihre Mitarbeit.
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Wigand, R. Hämagglutination durch Vaccinevirus. II. Erfahrungen mit der Hämagglutinationshemmung. Archiv f Virusforschung 7, 66–78 (1956). https://doi.org/10.1007/BF01253893
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DOI: https://doi.org/10.1007/BF01253893