Summary
Single cycle and multiple cycle growth of type 2 parainfluenza virus in stable amnion cells was examined. Hemadsorption plaque counts constituted a reliable method for quantitative estimation of infective virus. Virus from cells grown in serum-free medium supplemented with lactalbumin hydrolysate (LH) gave a consistently lower p.f.u./hemagglutinin ratio than virus from cells grown in medium containing calf serum. Infectivity titers, however, were the same in all media at the end of a single cycle of growth. Factors are discussed which might bear on the effect of LH on the production of virus in the presence or absence of calf serum. An unstable variant of type 2 parainfluenza virus is described which is characterized by the formation of hemadsorption plaques larger than those produced by 90% of the particles in stock preparations of virus. A strain of type 2 parainfluenza virus (clone 13) free of the large plaque variant was derived from stock cultures by limiting dilution and plaque purification techniques.
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DeVaux St. Cyr, C., andC. Howe: Immunochemica study of parainfluenza virus (type 2) in amnion cells. J. Bact.91, 1911 (1966).
Howe, C., C. Morgan, C. DeVaux St. Cyr, K. C. Hsu, andH. M. Rose: Morphogenesis of type 2 parainfluenza virus examined by light and electron microscopy. J. Virol.1, 215 (1967).
Reed, L. J., andH. Muench: A simple method of estimating fifty per cent endpoints. Amer. J. Hyg.27, 493 (1938).
Howe. C., L. T. Lee, andH. M. Rose: Collocalia mucoid: A substrate for myxovirus neuraminidase. Arch. Biochem.59, 512 (1961).
Darrell, R. W., andC. Howe: The neuraminidase of parainfluenza virus (type 2). Proc. Soc. exp. Biol. (N.Y.)116, 1091 (1964).
Hotchin, J. E., R. Deibel, andL. M. Benson: Location of noncytopathic myxovirus plaques by hemadsorption. Virology10, 275 (1959).
Deibel, R., andJ. Hotchin: Growth characteristics of hemadsorption virus type 1 (Myxovirus parainfluenza 3) in tissue culture. Virology14, 66 (1961).
Marston, R. Q., andE. R. Vaughan: Parainfluenza 3 — Assay and growth in tissue culture. Proc. Soc. exp. Biol. (N.Y.)104, 56 (1960).
Duc-Nguyen, H., andW. Henle: Replication of mumps virus in human leucocyte culture, J. Bact.92, 258 (1966).
French, E. L., andT. D. St. George: The growth and neutralization of Newcastle disease virus in BHK21 tissue cultures. Aust. J. exp. Biol. med. Sci.43, 371 (1965).
Reda, I. M., R. Rott, andW. Schäfer: Fluorescent antibody studies with NDV-infected cell systems. Virology22, 422 (1964).
Smorodintsev, A. A.: Experiences with the isolation and propagation of parainfluenza viruses. Acta virol.6, 338 (1962).
Sominina, A. A., Y. N. Zubzhitsky, andA. A. Smorodintsev: Dynamics of immunofluorescence and infectivity of parainfluenza 3 virus in susceptible cells. Acta virol.11, 424 (1967).
Zilka, E., L. Kawaklova, G. Vicari, andI. Archetti: Investigations of antiviral substances present in normal horse and calf sera. Arch. ges. Virusforsch.25, 177 (1968).
Milliken, S.: A delay in maturation as a cause of small plaque size with the NM strain of influenza A virus. J. gen. Virol.1, 189 (1967).
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Supported by grant No. AI 03168 from the National Institutes of Health, United States Public Health Service.
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Howe, C., Milliken, S.A. & Newcomb, E.W. Growth studies of parainfluenza virus (type 2). Archiv f Virusforschung 29, 50–62 (1970). https://doi.org/10.1007/BF01253880
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DOI: https://doi.org/10.1007/BF01253880