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Studies of laryngotracheitis virus in avian tissue cultures II. Deficient adsorption of a strain of laryngotracheitis virus

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Summary

In the plaque assay of the N71851 strain of laryngotracheitis virus (LTV) in chicken kidney (CK) monolayer cultures, the number of plaques which developed depended upon the treatment between adsorption and addition of agar overlay medium. Cultures in which the inoculum was aspirated after the adsorption period had a greater number of plaques than cultures in which the inoculum was aspirated and the cultures were washed. This effect was found to be due to a deficiency of virus to adsorb to cells. Under the conditions of the assay, the efficiency with which virus adsorbed to monolayers was too low to be measured. Therefore, the efficiency of the assay was also low. The initial association between virus and cells was easily disrupted by washing when adsorption was carried out at 5°C to prevent penetration. Treatment of cultures with diethylaminoethyl-dextran for one hour prior to inoculation increased the number of plaques in treated compared with untreated cultures by a factor of five.

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Author notes

  1. C. R. Rossi

    Present address: Department of Microbiology, School of Veterinary Medicine, Auburn University, 36830, Auburn, AL, USA

Authors and Affiliations

  1. Department of Veterinary Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA

    C. R. Rossi & A. M. Watrach

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  1. C. R. Rossi
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  2. A. M. Watrach
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Rossi, C.R., Watrach, A.M. Studies of laryngotracheitis virus in avian tissue cultures II. Deficient adsorption of a strain of laryngotracheitis virus. Archiv f Virusforschung 31, 313–320 (1970). https://doi.org/10.1007/BF01253766

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  • DOI: https://doi.org/10.1007/BF01253766

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