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Physicochemical studies of equine infectious anemia virus IV. Determination of the nucleic acid type in the virus

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Summary

The nucleic acid type in equine infectious anemia (EIA) virus was determined using isotope technique. In the present investigation, EIA virus grown in cultured horse leukocytes in the presence of3H-thymidine or3H-uridine was examined to see which precursor would be incorporated. The virus was purified from each infected culture by the combined procedure of ultracentrifugation, diethylaminoethyl cellulose chromatography and cesium chloride equilibrium density gradient centrifugation.3H-uridine was found to be incorporated into EIA virus, whereas3H-thymidine was not detected in the virus. Furthermore, purified3Huridine labeled EIA virus was fractionated by the Schmidt-Thannhauser-Schneider procedure. Radioactivity incorporated into the virus was mainly found in the fraction of ribonucleic acid (RNA). These results indicate that EIA virus contains RNA as the essential nucleic acid.

Physicochemical and biological characteristics of EIA virus which have thus far been established seem to closely resemble those of RNA containing tumor viruses, and visna and maedi viruses.

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Author notes

  1. Hideo Nakajima

    Present address: Equine Infectious Anemia Research Division, National Institute of Animal Health, Kodaira, Tokyo, Japan

Authors and Affiliations

  1. Equine Infectious Anemia Research Division, National Institute of Animal Health, Kodaira, Tokyo, Japan

    Shigeaki Tanaka & Chuzo Ushimi

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  1. Hideo Nakajima
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  2. Shigeaki Tanaka
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  3. Chuzo Ushimi
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Nakajima, H., Tanaka, S. & Ushimi, C. Physicochemical studies of equine infectious anemia virus IV. Determination of the nucleic acid type in the virus. Archiv f Virusforschung 31, 273–280 (1970). https://doi.org/10.1007/BF01253762

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  • DOI: https://doi.org/10.1007/BF01253762

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