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Flocculation of influenza virus by specific anti-neuraminidase antibody

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Summary

  1. 1.

    Flocculation of purified preparations of influenza virus has been demonstrated to occur in the presence of low dilutions of specific anti-neuraminidase sera. High dilutions of the sera caused microscopic aggregation of virions. It is suggested that the effects of anti-neuraminidase antibody on the replication of influenza virus observedin vitro could be caused by antibody binding virions to infected cells, rather than by inhibition of neuraminidase enzymic activity.

  2. 2.

    On examination in the electron microscope of virus floccules obtained with specific anti-neuraminidase sera, surface spikes were clearly seen on the virions. However, when influenza virus was similarly flocculated by serum containing antibodies that inhibited both haemagglutination and neuraminidase activities, the ends of the spikes were almost all completely obscured.

  3. 3.

    Measurement of the Michaelis constant (Km) of X-7 (F1) neuraminidase shows that the enzyme active site is related to that of A2/Singapore/57 neuraminidase.

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Author notes

  1. A. P. Kendal

    Present address: School of Public Health, University of Michigan, 48104, Ann Arbor, MI, USA

Authors and Affiliations

  1. Department of Bacteriology, University College Hospital Medical School, London

    A. P. Kendal & C. R. Madeley

  2. Department of Medical Microbiology, St. Thomas' Hospital Medical School, London, England

    A. P. Kendal & C. R. Madeley

Authors
  1. A. P. Kendal
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  2. C. R. Madeley
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Kendal, A.P., Madeley, C.R. Flocculation of influenza virus by specific anti-neuraminidase antibody. Archiv f Virusforschung 31, 219–229 (1970). https://doi.org/10.1007/BF01253756

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  • DOI: https://doi.org/10.1007/BF01253756

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