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Peptide composition of Sindbis virus variants

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Summary

The peptide composition of variants of Sindbis virus which differ in plaque-type, elution pattern from calcium phosphate columns and virulence in mice was analyzed. Differences in peptide number or composition were not detected in these variants. Since the Sindbis virion has an outer lipoprotein envelope which is derived at least in part from the plasma membrane of the host cell, peptide composition of purified chicken embryo membranes was analyzed. In contrast to the virions, the number of peptide units in the host cell membrane were too numerous to give resolution.

The chromatographic behavior of the hemagglutinating components of these plaque-type variants on calcium phosphate was also described. Unlike the intact virions, the hemagglutinating components obtained from these plaque-type variants eluted from the column at the same phosphate molarity. The elution pattern of the hemagglutinating components may be related to the removal of lipid from the virions or to the possible association of Tween-80 with the hemagglutinating protein.

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Author notes

  1. H. R. Bose

    Present address: Department of Microbiology, The University of Texas at Austin, Austin, Texas, USA

Authors and Affiliations

  1. Department of Microbiology, The University of Texas at Austin, Austin, Texas, USA

    M. A. Brundige, G. Z. Carl & B. P. Sagik

Authors
  1. H. R. Bose
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  2. M. A. Brundige
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  3. G. Z. Carl
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  4. B. P. Sagik
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Additional information

This investigation was supported by Grant GUDP 1598 from The National Science Foundation; Public Health Service Grant 1 R22 AI08516-01 from The Allergy and Infectious Disease Institute; and The Robert A. Welch Foundation Grant F-266.

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Bose, H.R., Brundige, M.A., Carl, G.Z. et al. Peptide composition of Sindbis virus variants. Archiv f Virusforschung 31, 207–214 (1970). https://doi.org/10.1007/BF01253754

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  • DOI: https://doi.org/10.1007/BF01253754

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