Summary
A method for concentrating and purifying enveloped viruses is described. Its salient feature is the use of an antihost immunoadsorbent column. Purified virus preparations were obtained in which viral-specific proteins per/mg total protein were enriched 800-fold, while host-specific complement fixing material became undetectable.
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This investigation was supported by Grant GUDP1598 from The National Science Foundation; Public Health Service Grant l R22 AI-08516-01 from the Allergy and Infectious-Disease Institute; and the Robert A. Welch Foundation Grant F-266.
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Bose, H.R., Carl, G.Z. & Sagik, B.P. Purification of two plaque variants of Sindbis virus. Archiv f Virusforschung 31, 200–206 (1970). https://doi.org/10.1007/BF01253753
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DOI: https://doi.org/10.1007/BF01253753