Summary
Two hitherto undescribed viruses were isolated from naturally-infected white clover plants.
One induced both cytoplasmic and intranuclear inclusions, and the other caused cytoplasmic inclusions of a new type, which we have called “Corner inclusion bodies.”
All three kinds of inclusion were found in the same cells.
Vital observations showed that the two kinds of cytoplasmic inclusion bodies developed principally from the plastids.
The intranuclear inclusions were shown to develop from the nucleoli.
Cytochemical tests showed that the cytoplasmic inclusions contained protein and ribonucleic acid.
On the basis of these findings, the possibility of virus synthesis in plastids and nucleoli is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Bald, J. G., 1948: The development of amoeboid inclusion bodies of tobacco mosaic virus. Australian J. Sci.B 1, 458–463.
—, 1949: A method for the selective staining of viruses in infected plant tissues. Phytopath.39, 395–402.
Basler, E., 1954: Relation between the nucleic acids of tobacco leaf and the biosynthesis of tobacco mosaic virus. Diss. Abstr.14, 1509–1510.
Bawden, F. C., 1950: Plant Viruses and Virus Diseases, 3rd Ed. Waltham, Mass. p. 45–46.
—, and B. Kassanis, 1941: Some properties of tobacco etch viruses. Ann. Appl. Biol.28, 107–118.
- and N. W. Pirie, 1953: Virus Multiplication Considered as a Form of Protein Synthesis, in: The Nature of Virus Multiplication (Fildes and Heyningen Eds). Cambridge, p. 21–45.
—, B. M. G. Hamlyn, and M. A. Watson, 1954: The distribution of viruses in different leaf tissues and its influence on virus transmission by aphids. Ann. Appl. Biol.41, 229–239.
Black, L. M., C. Morgan, and R. W. G. Wyckoff, 1950: Visualization of tobacco mosaic virus within infected cells. Proc. Soc. exper. Biol. a. Med. (Am.)73, 119–122.
Brandes, J., 1955: Elektronenmikroskopischer Nachweis von Pflanzenviren in ihren Wirtszellen. Naturw.42, 101.
Chantrenne, H., 1953: Problems of Protein Synthesis, in: The Nature of Virus Multiplication (Fildes and Heyningen Eds.). Cambridge, p. 1–20.
Clinch, P., 1932: Cytological studies of potato plants affected with certain virus diseases. Sci. Proc. Roy. Dublin Soc. n. s.20, 143–172.
Frey-Wyssling, A.: 1953: Submicroscopic Morphology of Protoplasm. Elsevier Publ. Company, p. 247.
Glick, D., 1949: Techniques of Histo- and Cyto-chemistry. Interscience Publ.
Hidaka, Z., 1955: Personal statement to the authors.
Holmes, F. O., 1948: The filterable viruses, in: Bergey's Manual of Determinative Bacteriology, 6th ed., William and Wilkins Co.
Kassanis, B., 1939: Intranuclear inclusions in virus-infected plants. Ann. Appl. Biol.26, 705–709.
Kausche, G. A., 1940: Ergebnisse und Probleme der experimentellen Virusforschung bei Pflanzen. Ber. dtsch. bot. Ges.58, 200–22.
—, and H. Ruska, 1940: Über den Nachweis des Tabakmosaikvirus in den Chloroplasten viruskranker Pflanzen. Naturw.28, 303–304.
Koshimizu, Y., 1955: Leucoplasts as an origin of intracellular inclusions and site of virus reproduction. Abstracts of papers presented at the Annual meeting of the Phytopath. Soc. Japan held on April 1955 (Japanese).
Leyon, H., 1953: Virus formation in chloroplasts. Exper. Cell Res.4, 362–370.
Lison, L., 1953: Histochemie et Cytochemie Animales (Japanese trans. by Imaizumi).
McWhorter, F. P., 1941 a: Isometric crystals produced byPisum virus 2 andPhaseolus virus 2. Phytopath.31, 760–761.
—, 1941 b: Plant-virus differentiation by trypanblue reactions within infected tissue. Stain Technol.16, 143–149.
—, and W. C. Price, 1949: Evidence that two different plant viruses can multiply simultaneously in the same cell. Science109, 116–117.
Rezende-Pinto, M., 1955: Sur les modifications de l'état colloïdal des chloroplastes. Brotéria (C. N.)24, 52–55.
—, and A. Lemos-Pereira, 1954: On the chloroplastonematic and optical structure ofClosterium chloroplasts. Portug. Acta Biol. (A)4, 1–4.
Rich, S., 1948a: The chemical nature and origin ofPhaseolus virus 2 crystalline inclusions. Science107, 194.
—, 1948b: A rapid stain method for detecting certain plant virus inclusions. Stain Technol.23, 19–20.
—, 1949: Some relations betweenPhaseolus virus 2 and its associated crystalline inclusions. Phytopath.39, 221–224.
Rischkov, V. L., and V. A. Smirnova, 1940: The tobacco mosaic virus content of tomato plastids. Mikrobiol.9, 170–181.
Sheffield, F. M. L., 1931: The formation of intracellular inclusions in solanaceous hosts infected with aucuba mosaic of tomato. Ann. Appl. Biol.18, 471–493.
Smith, K. M., 1933: Recent advances in the study of plant viruses. Churchill Co., p. 26–41.
Stahmann, M. A., and P. Kaesberg, 1955: Concepts and problems concerning the electron microscopy of plant viruses. Phytopath.45, 187–195.
Weber, F., I. Thaler, and G. Kenda, 1954: Die Piastiden derCleome-Epidermis. Österr. Bot. Z.102, 84–88.
Woods, M. W., and H. G. Du Buy, 1941: Synthesis of tobacco mosaic virus protein in relation to leaf chromoprotein and cell metabolism. Phytopath.31, 978–990.
—, and H. G. Du Buy, 1951: The action of mutant chondriogenes and viruses on plant cells with special reference to the plastids. Amer. J. Bot.38, 419–434.
—, and R. V. Eck, 1948: Nuclear inclusions produced by a strain of tobacco mosaic virus. Phytopath.38, 852–856.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koshimizu, Y., Iizuka, N. Origins and formation of intracellular inclusions associated with two leguminous virus diseases. Protoplasma 48, 113–133 (1957). https://doi.org/10.1007/BF01252890
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01252890