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Ultrastructural examination of phytohemagglutinin stimulated lymphocytes infected with vesicular stomatitis virus

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Summary

Human peripheral blood lymphocytes treated with PHA and subsequently infected with VSV supported extensive viral replication. Maximum titers of virus were obtained 16–24 hours after infection and represented several hundred fold increase over the input virus concentration. Untreated control cultures infected with VSV showed no significant viral increase. In electron micrographs of stimulated cultures infected with VSV, a large number of virions were produced by a few transforming cells of intermediate size. Viral penetration into the lymphocytes at the cell membrane, accompanied by cellular invagination, maturation at the marginal membrane surface, and release of virus to the outside by “budding” were consistent findings. The appearance of the nucleus in virus producing cells was similar to that in stimulated, uninfected cells. However, stimulated lymphocytes supporting extensive viral replication demonstrated some structural changes in the cytoplasm characterized by fewer mitochondria and polyribosomal aggregates and less active Golgi zones.

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Author notes

  1. I. L. Shechmeister

    Present address: Department of Microbiology, Southern Illinois University, 62901, Carbondale, IL, USA

Authors and Affiliations

  1. Clinical Laboratory #3N, U.S. Naval Hospital, 60085, Great Lakes, IL, USA

    G. K. McFadden

  2. Department of Microbiology, University of Notre Dame, 46556, Notre Dame, IN, USA

    R. L. Truitt

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  1. G. K. McFadden
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  3. I. L. Shechmeister
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McFadden, G.K., Truitt, R.L. & Shechmeister, I.L. Ultrastructural examination of phytohemagglutinin stimulated lymphocytes infected with vesicular stomatitis virus. Archiv f Virusforschung 41, 229–237 (1973). https://doi.org/10.1007/BF01252770

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  • DOI: https://doi.org/10.1007/BF01252770

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