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Stepwise degradation of poliovirus and top component by concentrated urea

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Summary

Poliovirus was inactivated by 7.2m urea at pH 7.0 and 37° C. The RNA was released intact. At temperatures not exceeding 40° C, the empty capsid underwent a further stepwise degradation, loosing VP-2 and VP-3 until a skeleton particle was left, consisting almost entirely of a mixture of VP-1 and 3.

Top component was disrupted with urea at high temperatures. At 62° C, VP-3a and VP-2 were gradually lost. Stable 50 S particles comprising all the VP-0 were formed; skeleton particles mainly composed of VP-1 and VP-3b were also formed, unless Mg++ was present.

The VP-1 band was resolved into two components. The structural role of the individual capsid polypeptides is discussed.

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Author notes

  1. D. Vanden Berghe & A. Boeyé

    Present address: Department of Biochemistry and Microbiology, Institute of Pharmacy, Vrije Universiteit Brussel, Belgium

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    1. D. Vanden Berghe
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    2. A. Boeyé
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    Vanden Berghe, D., Boeyé, A. Stepwise degradation of poliovirus and top component by concentrated urea. Archiv f Virusforschung 41, 216–228 (1973). https://doi.org/10.1007/BF01252769

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    • DOI: https://doi.org/10.1007/BF01252769

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