Summary
The synthesis of virus-specific RNA in Newcastle disease virus-infected cells is strongly suppressed by amino acid analogues (p-fluorophenylalanine, ethionine and canavanine) if they are present throughout the reproduction cycle or added 2–4 hours p.i. The analogues exert only a partial inhibitory effect when added 6 hours p.i. Virus-specific RNA synthesized in cells exposed to ethionine or canavanine has essentially the same sedimentational pattern as RNA formed in the absence of the analogues, whereas p-fluorophenylalanine preferentially inhibits 35S RNA accumulation. The content of “minus” strands in different RNA classes is not changed in p-fluorophenylalanine-treated cells. 50S RNA formation, although readily suppressed by cycloheximide was not inhibited by the amino acid analogues tested.
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Kaverin, N.V., Varich, N.L. & Smirnov, Y.A. Sedimentational pattern of virus-specific RNA synthesized in Newcastle disease virus-infected cells treated with amino acid analogues. Archiv f Virusforschung 41, 191–198 (1973). https://doi.org/10.1007/BF01252765
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DOI: https://doi.org/10.1007/BF01252765