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Differential growth of MHV(PRI) and MHV(C3H) in genetically resistant C3H mice rendered susceptible by eperythrozoon infection

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Summary

Mice of the C3H strain, which are genetically resistant to mouse hepatitis virus, MHV(PRI), became highly susceptible to that virus when preinfected with the murine blood parasite,Eperythrozoon coccoides (E. coccoides). Peak virus titers and deaths occurred 2 or more days later inE. coccoides-infected C3H mice than those events in genetically susceptible Princeton (PRI) mice. Growth curves and infectivity analyses of progeny virus in cultures of susceptible PRI and resistant C3H cells demonstrated that MHV(PRI) itself multiplied to high titer inE. coccoides- infected C3H mice. Variant virus, MHV(C3H), was also produced, but appeared later in the infection and comprised only a small fraction of the progeny virus. On subsequent passage of progeny virus inE. coccoides-infected C3H mice, MHV(PRI) continued to be produced far in excess of MHV(C3H). In normal (E. coccoides-free) C3H mice, progeny virus caused deaths or lesions indicative of the presence of variant virus, and the latter was recovered at a high titer. The action ofE. coccoides, whereby MHV(PRI) multiplication is initiated in genetically resistant (nonpermissive) C3H cells, could not be reproducedin vitro.

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Author notes

  1. G. C. Lavelle

    Present address: National Institute of Allergy and Infectious Diseases, Laboratory of Biology of Viruses, National Institutes of Health, 20014, Bethesda, MD, USA

Authors and Affiliations

  1. Department of Pathobiology, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland, USA

    G. C. Lavelle & F. B. Bang

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  1. G. C. Lavelle
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  2. F. B. Bang
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Lavelle, G.C., Bang, F.B. Differential growth of MHV(PRI) and MHV(C3H) in genetically resistant C3H mice rendered susceptible by eperythrozoon infection. Archiv f Virusforschung 41, 175–184 (1973). https://doi.org/10.1007/BF01252763

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  • DOI: https://doi.org/10.1007/BF01252763

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