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Differential sensitivity of virulent and attenuated strains of type 1 poliovirus to polyethylene glycol as a possible new genetic marker

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Summary

The effect of polyethylene glycol (PG) on poliovirus type 1 was studied by testing virulent (Mahoney) and attenuated (LSc2ab) virus strains as well as 20 isolates obtained from patients and vaccinees. PG was added in 2 per cent concentration to the agar overlay. Strains showing 20–50-fold inhibition of plaque formation were designated as PG-negative and those showing no inhibition as PG-positive. The virulent strains were found to be PG+, attenuated strains PG−-. Among virus isolates from the intestinal tract at various intervals after vaccination the PG character was found to be more stable than the intratypic antigenic (Ag) or Chromatographic (E) markers.

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Author notes

  1. V. F. Lokteva

    Present address: The U.S.S.R. AMS Institute of Poliomyelitis and Virus Encephalitides, Moscow, USSR

Authors and Affiliations

  1. The U.S.S.R. AMS Institute of Poliomyelitis and Virus Encephalitides, Moscow, USSR

    M. P. Chumakov, V. I. Zhevandrova & L. A. Shekoyan

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  1. V. F. Lokteva
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  2. M. P. Chumakov
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  3. V. I. Zhevandrova
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  4. L. A. Shekoyan
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Lokteva, V.F., Chumakov, M.P., Zhevandrova, V.I. et al. Differential sensitivity of virulent and attenuated strains of type 1 poliovirus to polyethylene glycol as a possible new genetic marker. Archiv f Virusforschung 41, 155–159 (1973). https://doi.org/10.1007/BF01252759

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  • DOI: https://doi.org/10.1007/BF01252759

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