Summary
Variations in the activity of catechol-O-methyltransferase (COMT) in peripheral organs and in the brain of rat and rabbit fetus during development have been studied. The pattern of changes in COMT activity in rat fetus differed to a great extent according to the respective organs studied. In kidney and liver sharp declines occurred between days 18 and 20 and days 16 and 18 of fetal life respectively, followed by progressive increases up to onset of birth. Brain COMT of rat fetus declined progressively from day 16 of fetal life up to 0 hours after birth, while COMT activity in adrenal and heart showed its maximum value at the 20th day of fetal life and at 0 hours after birth respectively. In the contrary, the developmental changes in activity of rabbit fetus were very similar in all the organs except in the adrenals, since it decreased between day 24 to 8 hours after birth in heart, liver, brain and kidney. In the adrenals an important increase could be seen between the 24th and 28th days of fetal life. Rat COMT activity during 4 and 8 hours of postnatal life in heart, liver and kidney declined from 0-hour value but it increased in the brain and adrenals. COMT in rabbit increased after birth in all the organs studied. The results suggest that metabolic degradation of catecholamines by 3-O-methylation in rat and rabbit fetus may have different developmental patterns to some extent according to the physiological status of the organ concerned. These species related differences for monoamine inactivation during fetal development may suggest a physiological role for COMT as a marker of the maturation of the autonomic nervous system.
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Agathopoulos, A. D., Nicolopoulos, N., Matsaniotis, N., Papadatos, C. Biochemical changes of catechol-O-methyltransferase during development of human liver. Pediatrics47, 125–128 (1971).
Axelrod, J.: Catechol-O-methyltransferase in rat liver. In: Methods in Enzymology (Colowick, S. P., Kaplan, N. O., eds.), Vol. 5, pp. 748 to 751. London: Academic Press.
Axelrod, J., Senoh, J., Withop, B. O-methylation of catecholamines in vivo. J. Biol. Chem.233, 697–705 (1958).
Castren, O., Kaartinen, N., Saarikoski, S. The metabolism of3H-noradrenaline in human placenta and fetal tissue homogenates. Ann. Chir. Gynec. Fenn.57, 405–409 (1968).
Castren, O., Saarikoski, S. The effect of some different factors on the metabolism of3H-noradrenaline in placental, adrenal and fetal tissue homogenates. Acta Obstet. Gynec. Scand.48, Supp. 3, 151–157 (1969).
Gennser, G., von Studnitz, W. Monoamine oxidase and catechol-O-methyltransferase activity in umbilical vessels of the human fetus. Experientia25, 980–981 (1969).
Gennser, G., Rosengren, E., von Studnitz, W. Distribution of nor-adrenaline and of monoamine oxidase and catechol-O-methyltransferase activity in human fetal heart. Experientia29, 20–22 (1972).
Ignarro, L. J., Shideman, F. E. Catechol-O-methyltransferase and monoamine oxidase activities in the heart and liver of the embryonic and developing chick. J. Pharmacol. Exp. Ther.159, 29–37 (1968).
Iversen, L. L., De Champlain, J., Glowinski, J., Axelrod, J. Uptake storage and metabolism of norepinephrine in tissues of the developing rat. J. Pharmacol. Exp. Ther.157, 509–516 (1967).
Kopin, I. J. Storage and metabolism of catecholamines: Role of monoamine oxidase. Pharmacol. Rev.16, 179–191 (1964).
Saarikoski, S.: Fate of noradrenaline in the human foetoplacental unit. In vivo studies on placental transfer, metabolism and distribution in foetal tissues. Acta Physiol. Scand. Suppl.421 (1974).
Sandler, M., Ruthven, C. R. J., Contractor, S. F., Wood, C., Booth, R. T., Pinkerton, J. H. M. Transmission of noradrenaline across the human placenta. Nature197, 598 (1963).
Saarikoski, S., Castren, O. Distribution and metabolism of3H-noradrenaline in various tissues of the human fetus in the fetoplacental unit. Acta Obstet. Gynecol. Scand.50, Suppl. 9, 60 (1971).
Parvez, H., Parvez, S. Microradioisotopic determination of enzymes catechol-O-methyltransferase, phenylethanolamine-N-methyltransferase and monoamine oxidase in a single concentration of tissue homogenate. Clin. Chim. Acta46, 85–90 (1973).
Parvez, H., Parvez, S. The role of hypophyseo-adrenocortical system in the regulation of enzyme monoamine oxidase in rabbit fetuses. Biochem. Biophys. Res. Commun.50, 901–907 (1973).
Parvez, S., Parvez, H. Placental transfer of3H-epinephrine and its metabolites to the fetal heart during variable hormonal treatments. Hormone Res.5, 321–330 (1974).
Parvez, H., Parvez, S. The effects of foetal decapitation on the antenatal development of the enzymes monoamine oxidase and catechol-O-methyltransferase in rabbit foetuses. Biochim. Biophys. Acta338, 21–28 (1974).
Parvez, H.: Influence des hormones glucocorticoïdes sur la sécrétion et l'excrétion urinaire des catécholamines et sur les activités enzymatiques monoamine oxydase et catéchol-O-méthyltransférase. Doctor of Science Thesis, Paris University No. 1185 (1973).
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Parvez, H., Ismahan, G., Parvez, S. et al. Developmental changes in the activity of catechol-O-methyl transferase in rat and rabbit fetuses. J. Neural Transmission 44, 65–75 (1979). https://doi.org/10.1007/BF01252702
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DOI: https://doi.org/10.1007/BF01252702