Summary
The time-related changes of dopamine (DA) and norepinephrine (NE) pools were investigated in heart, superior cervical ganglion (SCG) and urine in rats treated chronically with guanethidine (50 mg/kg i.p. five days each week). The efficiency of sympathectomy was assessed by the great loss of NE in heart and superior cervical ganglion (SCG) (−96% and −76% respectively of control values on day 18) together with the ready reduction of NE and 3-methoxy-4-hydroxyphenylglycol (MHPG) in urine.
The pattern of changes was quite different for DA, which was less readily affected and at a lesser extent than NE in heart and SCG thus suggesting the presence of norepinephrine-independent DA stores. Similarly the urinary excretion of free DA, free 3, 4-dihydroxyphenylacetic acid (DOPAC) and free homovanillic acid (HVA) was slightly decreased only from the 9th day, whereas urinary conjugated DA remained unaltered. These results indicate that the greatest portion of urine free and conjugated DA, free DOPAC and free HVA derives from peripheral pools located outside noradrenergic neurons. Alternatively, the time-course of DA sensitivity to guanethidine suggests that a portion of urine DA may originate from DA stored independently from NE in noradrenergic neurons.
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Adam WR, Adams BA (1985) Production and excretion of dopamine by the isolated perfused rat kidney. Renal Physiol 8: 150–158
Anton AH, Sayre DF (1962) A study of the factors affecting the aluminium oxyde-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther 138: 360–375
Bell C (1982) Dopamine as a postganglionic autonomie neurotransmitter. Neuroscience 7: 1–8
Bell C, Lang WJ, Laska F (1978a) Dopamine-containing vasomotor nerves in the dog kidney. J Neurochem 31: 77–83
Bell C, Lang WJ, Laska F (1978b) Dopamine-containing axons supplying the arterio-venous anastomoses of the canine paw pad. J Neurochem 31: 1329–1333
Bjorklund A, Cegrell L, Falck B, Ritzen M, Rosergren E (1970) Dopamine-containing cells in sympathetic ganglia. Acta Physiol Scand 78: 334–338
Boudet C, Peyrin L, Tavitian E, Claustre J, Favre R (1984) Studies on the central or peripheral origin of free and sulfated 3,4-dihydroxyphenylacetic acid in rat plasma. Eur J Pharmacol 103: 295–301
Burnstock G, Evans B, Gannon BJ, Heath JW, James V (1971) A new method of destroying adrenergic nerves in adult animals using guanethidine. Br J Pharmacol 43: 295–301
Collier B, Johnson G, Quik M, Weiner S (1984) Effect of chemical destruction of adrenergic neurons on some cholinergic mechanisms in adult rat sympathetic ganglia. Br J Pharmacol 82: 827–832
Cottet-Emard JM, Peyrin L, Bonnod J (1980) Dietary induced changes in catecholamine metabolites in rat urine. J Neural Transm 48: 189–201
Dinerstein RJ, Vannice J, Henderson RC, Roth LJ, Goldberg JL, Hoffman PC (1979) Histofluorescence techniques provide evidence for dopamine-containing neuronal elements in canine kidney. Science 205: 497–499
Edwards DJ, Ravitch J (1983) Origin of acidic and neutral urinary catecholamine metabolites in rats. Prog Neuropsychopharmacol Biol Psychiatry [Suppl] abstr no S 82
ErÄnkö O (1978) Small intensely fluorescent (SIF cells) and nervous transmission in sympathetic ganglia. Ann Rev Pharmacol Toxicol 18: 417–430
ErÄnkö L, ErÄnkö O (1971) Effect of guanethidine on nerve cells and small intensely fluorescent cells in sympathetic ganglia of newborn and adult rats. Acta Pharmacol Toxicol 30: 403–416
Evans BK, Singer G, Armstrong S, Saunders PE, Burnstock G (1975) Effects of chronic intracranial injection of low and high concentrations of guanethidine in the rat. Pharmacol Biochem Behav 3: 219–228
Favre R, De Haut M, Dalmaz M, Pequignot JM, Peyrin L (1986) Peripheral distribution of free dopamine and its metabolites in the Rat. J Neural Transm 66: 135–149
Fredholm BB, Farmbo LO, Hamberger B (1979) Plasma catecholamines, cyclic AMP and metabolic substrates in hemorragic shock of the rat. The effect of adrenal demedullation and 6-hydroxydopamine treatment. Arch Physiol Scand 108: 481–495
Fried G, Lagercrantz H, Hökfelt T (1978) Improved isolation of small noradrenergic vesicles from rat seminal ducts following castration. A density gradient centrifugation and morphological study. Neuroscience 3: 1271–1291
Fried G, Lagercrantz H, Klein R, Thureson-Klein A (1984) Large and small noradrenergic vesicles-origin contents and functional significance. Catecholamines part A: basic and peripheral mechanisms. In: Usdin E, Carlsson A, Dahlström MA, Engel J (eds) Neurology and neurobiology, vol 8 A. Alan R Liss, New York, pp 45–53
Fujita T (1977) Concept of paraneurons. Arch Histol Jpn 40, 1–12
Grobecker H, Roizen MF, Jacobowitz DM, Kopin IJ (1977) Effect of prolonged treatment with adrenergic neuron blocking drugs on sympathoadrenal reactivity in rats. Eur J Pharmacol 46: 125–133
Grobecker H, Roizen MF, Kopin IJ (1977) Effect of tyramine and guanethidine on dopamine-beta-hydroxylase activity and norepinephrine concentrations in vesicular fraction of the heart and plasma of rats. Life Sci 20: 1009–1015
Hanbauer I, Hellström S (1978) The regulation of dopamine and noradrenaline in the rat carotid body and its modification by denervation and by hypoxia. J Physiol (Lond) 282: 21–34
Heath JW, Evans BK, Gannon GJ, Burnstock G, James VB (1972) Degeneration of adrenergic neurons following guanethidine treatment: an ultrastructural study. Virchows Arch [Cell Pathol] 11: 182–197
Heym Ch, Addicks K, Gerold N, Schröder H, König R, Wedel Ch (1980) Catecholamines in paraganglionic cells of the rat superior cervical ganglion: functional aspects. Adv Biochem Psychopharmacol 25: 87–94
Hoeltdke RD, Wurtman RJ (1974) Synthesis of DOPA in rat stomach following ingestion of cereals. Metabolism 23: 25–31
Jensen-Holm J, Juul P (1970) The effects of guanethidine, pre- and postganglionic nerve division on the rat superior cervical ganglion: cholinesterases and catecholamines (histochemistry) and histology. Acta Pharmacol Toxicol 28: 283–298
Johnson EM, O'Brien F (1976) Evaluation of the permanent sympathectomy produced by the administration of guanethidine to adult rats. J Pharmacol Exp Ther 196: 53–61
Kuchel O, Buu NT, Unger Th (1979) Free and conjugated dopamine: physiological and clinical implications. In: Imbs JL, Schwartz J (eds) Peripheral dopamine receptors. Pergamon Press, Oxford
Kvetnansky R, Weise VK, Thoa NB, Kopin IJ (1979) Effects of chronic guanethidine treatment and adrenal medullectomy on plasma levels of catecholamines and corticosterone in forcibly immobilized rats. J Pharmacol Exp Ther 209: 287–291
Lackovic Z, Relja M, Neff NH (1981) Evidence for a peripheral dopaminergic neural system. In: Usdin E, Weiner N, Youdin MBH (eds) Function and regulation of monoamine enzymes. Basic and clinical aspects. Macmillan, New York, pp 361–370
Landsberg L, Berardino MB, Stoff J, Young JB (1978) Further studies on catechol uptake and metabolism in rat small bowel in vivo: (1) a quantitatively significant process with distinctive structural specification; and (2) the formation of a dopamine glucuronide reservoir after chronic L-DOPA feeding. Biochem Pharmacol 27: 1365–1371
Lishajko F (1968) Occurence and some properties of dopamine containing granules in the sheep adrenal. Acta Physiol Scand 72: 255–256
Nelson DL, Molinoff PB (1976) Distribution and properties of adrenergic storage vesicles in nerve terminals. J Pharmacol Exp Ther 196: 346–359
Orloff LA, Orloff MS, Bunnet NW, Walsh JH (1985) Dopamine and norepinephrine in the alimentary tract: changes after chemical sympathectomy and surgical vagotomy. Life Sei 36: 1625–1631
Pequignot JM, Cottet-Emard JM, Dalmaz Y, De Haut De Sigy M, Peyrin L (1986) Biochemical evidence for norepinephrine stores outside the sympathetic nerves in rat carotid body. Brain Res 367: 238–243
Peyrin L, Cottet-Emard JM, Javoy F, Agid Y, Herbet A, Glowinski J (1978) Long-term effects of unilateral 6-hydroxydopamine destruction of the dopaminergic nigrostriatal pathway on the urinary excretion of catecholamines (dopamine, norepinephrine, epinephrine) and their metabolites. Brain Res 143: 567–572
Snider SR, Almgren O, Carlsson A (1973) The occurrence and functional significance of dopamine in some peripheral adrenergic nerves of the rat. Naunyn Schmiedebergs Arch Pharmacol 278: 1–12
Soares Da Silva P, Davidson R (1985) Effects of 6-hydroxydopamine on dopamine and noradrenaline content in dog blood vessels and heart. Evidence for a noradrenaline-independent dopamine pool. Naunyn Schmiedebergs Arch Pharmacol 329: 253–257
Snider SR, Kuchel O (1983) Dopamine: an important neurohormone of the sympathoadrenal system. Significance of increased peripheral dopamine release for the human stress response and hypertension. Endocr Rev 4: 291–309
Stephenson RK, Sole MJ, Baines AD (1982) Neural and extraneural catecholamine production by rat kidneys. Am J Physiol 242: F261-F266
Unger T, Buu NT, Kuchel O (1980) Conjugated DA: peripheral origin, distribution and response to acute stress in the dog. Can J Physiol Pharmacol 58: 22–27
Van Loon GR (1983) Plasma dopamine: regulation and significance. Fed Proc 42: 3012–3018
Wang PC, Buu NT, Kuchel O, Genest J (1983) Conjugation patterns of endogenous plasma catecholamines in human and rat. A new specific method for analysis of glucuronide-conjugated catecholamines. J Lab Clin Med 101: 141–151
Westerink BHC, Mulder TBA (1981) Determination of picomole amounts of dopamine, noradrenaline, 3,4-dihydroxyphenylalanine, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and 5-hydroxyindoleacetic acid in nervous tissue after one step purification on Sephadex G 10 using high performance liquid chromatography with a novel type of electrochemical detection. J Neurochem 36: 1449–1462
Wood JM (1980) Neurobiology of cerebrospinal fluid. Plenum Press, New York
Yoneda S, Tomioka H, Fukuyama M, Lee L, Iyota I, Okajima H, Inoue A, Sasaki S, Takeda K, Takahashi H, Yoshimura M, Nakagawa M, Ijichi H (1985) Peripheral origin of plasma dopamine. Jpn CircJ 49: 1028–1034
Zimmermann H (1979) Vesicle recycling and transmitter release. Neuroscience 4: 1773–1803
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Favre, R., De Haut, M., Boudet, C. et al. Differential effect of guanethidine on dopamine and norepinephrine pools in urine, heart and superior cervical ganglion in the rat. J. Neural Transmission 70, 19–37 (1987). https://doi.org/10.1007/BF01252506
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DOI: https://doi.org/10.1007/BF01252506