Abstract
Capillary zone electrophoresis was investigated for the separation of free fatty acids as an alternative to well established techniques such as GC or HPLC. The analysis was performed with indirect UV detection in a counterelectroosmotic flow mode using a diethylbarbiturate carrier electrolyte at a pH between 10 and 11 in a mixed aqueous-organic solvent. Separation of saturated and unsaturated fatty acids could be achieved after bromination of the double bonds. Problems with wall adsorption of fatty acids could be overcome by increasing the temperature and using a high concentration of a zwitterionic reagent to inactivate the silica surface. Increased sensitivity could be achieved after preconcentration on alumina. The method was applied to the determination of free fatty acids in dairy products. The advantages compared to traditional methods include short analysis times and simple preparation steps.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
C. de Jong, H. T. Badings,J. High Resolut. Chrom. 1990,13, 94.
K. Helrich (ed.),Official Methods of Analysis of the Association of Official Analytical Chemistry, 15th Ed., Vol. 2, AOAC, Arlington, VA, 1990, pp. 963–965.
M. C. Martin-Hernandez, L. Alonso, M. Juarez, J. Fontecha,Chromatographia 1988,25, 87.
J. W. King, E. C. Adams, B. A. Bidlingmeyer,J. Liq. Chromatogr. 1982,5, 275.
Y. Tsuyama, T. Uchida, T. Goto,J. Chromatogr. 1992,596, 181.
J. F. Lawrence, C. F. Charbonneau,J. Chromatogr. 1988,445, 189.
H. Kawasaki, N. Maeda, H. Yuki,J. Chromatogr. 1990,516, 450.
H. D. Durst, M. Milano, E. J. Kikta, S. A. Connelly, E. Grushka,Anal. Chem. 1975,47, 1797.
H. C. Jordi,J. Liq. Chromatogr. 1978,1, 215.
H. Miwa, M. Yamamoto, T. Nishida, K. Nunoi, M. Kikuchi,J. Chromatogr. 1987,416, 237.
P. R. Brown, J. M. Beebe, J. Turcotte,Crit. Rev. Anal. Chem. 1989,21, 193.
Y. Yasaka, M. Tanaka,J. Chromatogr. B 1994,659, 139.
J. Romano, P. Jandik, W. R. Jones, P. E. Jackson,J. Chromatogr. 1991,546, 411.
H. Engelhardt, W. Beck, T. Schmitt,Kapillarelektrophorese, Vieweg, Braunschweig, 1994, p. 96.
W. R. Jones, P. Jandik,J. Chromatogr. 1991,546, 445.
M. M. Bushey, J. W. Jorgenson,J. Chromatogr. 1989,480, 301.
C. Schwer, E. Kenndler,Anal. Chem. 1991,63, 1801.
H. C. Deeth, C. H. Fitz-Gerald, A. J. Snow,N. Z. J. Dairy Sci. Technol. 1983,18, 13.
I. Hornstein, J. A. Alford, L. E. Elliott, P. F. Crowe,Anal. Chem. 1960,32, 540.
E. Ballesteros, S. Cardenas, M. Gallego, M. Valcarcel,Anal. Chem. 1994,66, 628.
Author information
Authors and Affiliations
Additional information
Dedicated to Univ. Prof. Dr. Karl Winsauer on the occasion of his 70th birthday
Rights and permissions
About this article
Cite this article
Buchberger, W., Winna, K. Determination of free fatty acids by capillary zone electrophoresis. Mikrochim Acta 122, 45–52 (1996). https://doi.org/10.1007/BF01252404
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01252404