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Acridine orange fluorescence in cell physiology, cytochemistry and medicine

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Summary

  1. 1.

    The article reviews history and theory of acridine orange fluorescence microscopy in application to (i) vital staining, (ii) fixed preparations.

  2. 2.

    Acridine orange as a cytotopochemical reagent for nucleic acids (DNA and RNA) is discussed in detail. This has especially been applied (i) to cytodiagnosis of cancer; (ii) in virus research; and (iii) in cell physiology.

  3. 3.

    The physicochemical and cytochemical bases of the acridine orange fluorescence technique after Bertalanffy et al. are presented in detail.

  4. 4.

    Correlations between RNA content, protein synthesis and cell renewal are demonstrated by the acridine orange technique.

  5. 5.

    Effects of radiation on cytoplasmic RNA are discussed with respect to clinical and experimental observations and their implications.

  6. 6.

    Acridine orange fluorescence leads to new concepts in the problem and theory of cancer. It emphasizes the possible role of cytoplasmic RNA (basophilia, ribosomes) in malignant growth. Recent facts and theories are discussed in view of the concept that cytoplasmic changes may play a more fundamental role in carcinogenesis than hitherto assumed.

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References

  • Altmann. H. W., 1955: Allgemeine morphologische Pathologie des Cytoplasmas. Die Pathobiosen. Handb. d. Allg. Pathol., herausgeg. von F. Büchner, E. Letterer und F. Roulet. Bd. II/1, 419.

  • Anderson, E. S., 1957: Visual observation of deoxyribonucleic acid changes in bacteria during growth of bacteriophage. Nature (Brit.)180, 1336.

    Google Scholar 

  • —, J. A. Armstrong, and J. S. F. Niven, 1959: Fluorescence microscopy: Observation of virus growth with aminoacridines. Symposia Soc. Gen. Microbiol.9, 224.

    Google Scholar 

  • Armstrong, J. A., 1956: Histochemical differentiation of nucleic acids by means of induced fluorescence. Exper. Cell Res.11, 640.

    Google Scholar 

  • — and J. S. F. Niven, 1957: Histochemical observations on cellular and virus nucleic acids. Nature (Brit.)180, 1335.

    Google Scholar 

  • Austin, C. R., and M. W. H. Bishop, 1959: Differential fluorescence in living rat eggs treated with acridine orange. Exper. Cell Res.17, 35.

    Google Scholar 

  • Bayreuther, K., 1960: Chromosomes in primary neoplastic growth. Nature (Brit.)186, 6.

    Google Scholar 

  • Benaim Pinto, V., e H. Caballero, 1962: Citología ginecológica de fluorescencia (Metodo de Bertalanffy). Acta Cientif. Venezolana13, 65.

    Google Scholar 

  • Bertalanffy, F. D., 1960 a: Fluorescence microscopy for the rapid diagnosis of malignant cells by exfoliative cytology. Mikroskopie15, 67.

    Google Scholar 

  • —, 1960b: Fluorescence microscopy for cytodiagnosis of cancer. Postgraduate Med.28, 627.

    Google Scholar 

  • —, 1961: Exfoliative Krebsdiagnose mit Akridinorange-Fluoreszenzmikroskopie. Krebsarzt16, 521.

    PubMed  Google Scholar 

  • —, 1962a: Cytodiagnosis of body effusions by fluorescence microscopy. Arch. Pathology73, 333.

    Google Scholar 

  • —, 1962b: Evaluation of the acridine-orange fluorescence microscope method for cytodiagnosis of cancer. Ann. N. Y. Acad. Sci.93, art. 16, 715.

    Google Scholar 

  • —, 1963: Cell renewal as the basis of diagnostic exfoliative cytology. Amer. J. Obstetr.85, 383.

    Google Scholar 

  • —, and Ch. Lau, 1962a: Cell renewal. Internat. Rev. Cytol.13, 357.

    Google Scholar 

  • —, 1962b: Rates of cell division of transplantable malignant rat tumors. Cancer Res.22, 627.

    PubMed  Google Scholar 

  • —, and Ch. Lau, 1962c: Mitotic rate and renewal time of the corneal epithelium of the rat. Arch. Ophthalm.68, 546.

    Google Scholar 

  • Bertalanffy, F. D., and K. P. Nagy, 1962: Fluorescence microscopy and photography with acridine orange. Med. Radiogr. Photogr. (Eastman Kodak Co., Rochester, N.Y.)38, 82.

    Google Scholar 

  • Bertalanffy, L. von, 1951: Theoretische Biologie.2. Bd.: Stoffwechsel, Wachstum. 2. Aufl. Bern.

  • - 1953: Biophysik des Fließgleichgewichts. Übers, von W. W. Westphal. Braunschweig.

  • —, 1959a: Eine fluoreszenzmikroskopische Schnellmethode zur Diagnose des gynäkologischen Carcinoms. Klin. Wschr.37, 469.

    PubMed  Google Scholar 

  • —, 1959b: Radiation cell changes. Acta Cytol.3, 354, 361.

    Google Scholar 

  • - 1963: Cytodiagnosis of cancer by fluorescence microscopy. Annales d'Histochimie. In Press.

  • - et al., 1957 (Dec.): Fluorescence microscopy in cancer research. AMA Clinical Meeting, Philadelphia.

  • — and F. D. Bertalanffy, 1960: A new method for cytological diagnosis of pulmonary cancer. Ann. N. Y. Acad. Sci.84, art. 5, 225.

    Google Scholar 

  • —, 1961: Die Fluoreszenzmethode in der Zytodiagnostik des gynäkologischen Karzinoms. Med. Welt35, 1742.

    Google Scholar 

  • —, 1962: Akridinorange-Fluoreszenz-Cytodiagnostik in der Früherkennung des Krebses. Ärztl. Mitt.47/59, 2393.

    Google Scholar 

  • — and I. Bickis, 1956: Identification of cytoplasmic basophilia (ribonucleic acid) by fluorescence microscopy. J. Histochem. a. Cytochem.4, 481.

    Google Scholar 

  • —, F. Masin, and M. Masin, 1956: Use of acridine-orange fluorescence technique in exfoliative cytology. Science124, 1024.

    PubMed  Google Scholar 

  • —, M. Masin, and F. Masin, 1958: A new and rapid method for diagnosis of vaginal and cervical cancer by fluorescence microscopy. Cancer11, 873.

    PubMed  Google Scholar 

  • Bickis, I., R. R. Estwick, and J. S. Campbell, 1956: Observations on initiation of skin carcinoma in pituitary dwarf mice. I. Cancer9, 763.

    PubMed  Google Scholar 

  • Bishop, M. W. H., and C. R. Austin, 1957: Mammalian spermatozoa. Endeavour16, 137.

    Google Scholar 

  • Bontke, E., G. Kern und N. Schümmelfeder, 1960: Die Akridinorange-Fluorochromierung in der gynäkologischen Zytodiagnostik. Geburtshilfe u. Frauenheilkunde20, 24.

    Google Scholar 

  • Boyle, R. E., et al., 1962: The interaction of deoxyribonucleic acid and acridine orange. Arch. Biochem. Biophys.96, 47.

    PubMed  Google Scholar 

  • Brachet, J., 1940: La détection histochimique des acides pentosenucléiques. C. r. soc. biol.133, 88.

    Google Scholar 

  • - 1957: Biochemical Cytology. New York.

  • Bradley, D. F., 1961: Molecular biophysics of dye-polymer complexes. Trans. N.Y. Acad. Sci.24, 64.

    Google Scholar 

  • Cameron, D. E., 1962: Stable RNA devised for large-dose aid to memory of aged. Report. Med. TribuneSept. 3, 21.

    Google Scholar 

  • Carelli, E., R. Garbagni, e M. Fazio, 1961: La microscopia a fluorescenza nella citodiagnosi dei tumori bronco-polmonari. Minerva Med.52, 4242.

    PubMed  Google Scholar 

  • Caspersson, T., 1941: Studien über den Eiweißumsatz der Zelle. Naturwiss.29, 33.

    Google Scholar 

  • —, and L. Santesson, 1942: Studies on protein metabolism in the cells of epithelial tumors. Acta radiol., Suppl.42, 1.

    Google Scholar 

  • Choné, B., 1962: DreidimensionaleTumorzelldiagnostik. Münch. Med. Wschr.104, 621.

    Google Scholar 

  • Cowdry, E. V., 1955: Cancer Cells. 2nd ed. Philadelphia and London.

  • Dart, L. H., Jr., and T. R. Turner, 1959: Fluorescence microscopy in exfoliative cytology. Lab. Investigation8, 1513.

    Google Scholar 

  • De Bruyn, P. P. H., et al., 1953: In vivo and in vitro affinity of diaminoacridines for nucleoproteins. Exper. Cell Res.4. 174.

    Google Scholar 

  • De Lerma, B., 1958: Die Anwendung von Fluoreszenzlicht in der Histochemie. Handb. d. Histochemie, herausgeg. von W. Graumann und K. Neumann. Bd. I/1,78.

  • Donáth, T., and I. Lengyel, 1961: Analysis of the staining properties of acridine orange in epithelial cells. Acta Morphol. Acad. Scient. Hungar.10, 21.

    Google Scholar 

  • Droz, B., and C. P. Leblond, 1962: Migration of proteins along the axon of the sciatic nerve. Science137, 1047.

    PubMed  Google Scholar 

  • Druckrey, H., 1951: Experimentelle Beiträge zum Mechanismus der cancerogenen Wirkung. Arzneimittelforsch. 383.

  • Eichner, D., 1959a: Fluoreszenzmikroskopische Untersuchungen am Duodenalepithel der weißen Maus (Mus musculus). Z. Zellforsch.49, 418.

    Google Scholar 

  • - 1959b: Experimentelle Untersuchungen zur Vital- und Supravital-Fluoreszenzmikroskopie mit Akridinorange. Verh. Anat. Ges. 56. Versammlung. 420.

  • Epstein, M. A., 1960: Constitution of tumor viruses. Nat. Cancer Inst. (U.S.A.), Monograph4, 53.

    Google Scholar 

  • - 1961: The electron microscopy of viruses. Proc. Roy. Soc. Medic. 1071.

  • Goessner, W., 1949: Zur Histochemie des Strugger-Effekts. Verh. dtsch. Ges. Pathol.33, 102.

    Google Scholar 

  • Grubb, C., and J. G. S. Crabbe, 1961: Fluorescence microscopy in exfoliative cytology. Brit. J. Cancer15, 483.

    PubMed  Google Scholar 

  • Gurr, E., 1962: Staining-Practical and Theoretical. London and Baltimore.

  • Haitinger, M., 1938, 1960: Fluoreszenz-Mikroskopie, 1., 2. Aufl. Leipzig.

  • Hamperl, H., und N. Schümmelfeder. 1961: Über Fluoreszenzmikroskopie. Ciba-Symposium9, 50.

    Google Scholar 

  • Harbers, E., 1960: Wirkungen ionisierender Strahlen auf die Nukleinsäuren. Strahlentherapie112, 333.

    PubMed  Google Scholar 

  • Hasselmann, H., und D. Wittekind, 1957: Phasenkontrast-Fluoreszenz-Mikroskopie. Z. wiss. Mikrosk.63, 216.

    PubMed  Google Scholar 

  • Herovici, C., 1959: Radiation cell changes. Acta Cytol.3, 360.

    Google Scholar 

  • Hitchcock, C. R., and S. L. Scheiner, 1961: Early diagnosis of gastric cancer. Surg., Gyn., Obstetr.113, 665.

    Google Scholar 

  • Höfler, K., 1947: Was lehrt die Fluoreszenzmikroskopie von der Plasmapermeabilität und Stoffspeicherung? Mikroskopie2, 13.

    Google Scholar 

  • —, 1949: Fluoreszenzmikroskopie und Zellphysiologie. Biologia Generalis19, 90.

    Google Scholar 

  • Howatson, A. F., and A. W. Ham, 1955: Electron microscope study of sections of two rat liver tumors. Cancer Res.15, 62.

    PubMed  Google Scholar 

  • Hunter, D. T., Jr., and N. Brown, 1961: Morphology of benign cells as observed through the acridine orange fluorescence technic. Acta Cytol.5, 250.

    PubMed  Google Scholar 

  • Hydén, H., 1955: Nucleic acids and proteins. Neurochemistry, ed. by K. A. C. Elliott, I. H. Page, and J. H. Quastel. Springfield, Ill., 227.

  • —, 1961: Altered RNA seen to affect memory. Report. Med. TribuneFebr. 27, 1.

    Google Scholar 

  • VIII International Cancer Congress, 1962: Abstracts of papers. Moscow.

  • Jackson, J. F., 1961: Supravital blood studies, using acridine orange fluorescence. Blood17, 643.

    PubMed  Google Scholar 

  • —, 1962: Histochemical identification of megakaryocytes from peripheral blood examined for tumor cells. Cancer15, 259.

    PubMed  Google Scholar 

  • Kaplan, H. S., 1959: Some implications of indirect induction mechanisms in carcinogenesis: A review. Cancer Res.19, 791.

    Google Scholar 

  • Kinzel, H., und E. Bolay, 1961: Über die diagnostische Bedeutung der Entmischungs- und Fällungsformen bei Vitalfärbung von Pflanzenzellen. Protoplasma54, 179.

    Google Scholar 

  • Kornfield, H. J., and A. A. Werder, 1960: A differential nucleic acid fluorescent stain applied to cell culture systems. Cancer13, 458.

    PubMed  Google Scholar 

  • Kosenow, W., 1955: Lebende Blutzellen im Fluoreszenz- und Phasenkontrast-mikroskop. Bibliotheca Haematologica4, 1.

    PubMed  Google Scholar 

  • Kurnick, N. B., 1955: Histochemistry of nucleic acids. Intern. Rev. Cytol.4, 221.

    Google Scholar 

  • La Bonte, R. E., 1961: Evaluation of acridine orange fluorescence microscopy and the Papanicolaou technique. Lab. Digest24, No. 7, 8.

    Google Scholar 

  • Liu, W., 1961: Fluorescence microscopy in exfoliative cytology. I, II. Arch. Pathology71, 282, 286.

    Google Scholar 

  • Marmont, A., 1959a: Nueleolytic phagocytosis (LE cell phenomenon) in systemic lupus erythematosus, rheumatoid arthritis and systemic scleroderma. Immunopathology, 1st Internat. Symposium, Basel, 479.

  • - 1959b: Acridine orange fluorescence microscopy in haematology. 7th Congress European Soc. Haematology, London, 7.

  • Marquez-Monter, H., y G. Navarro, 1961: Microscopia fluorescente en citologia exfoliativa cervico vaginal. Rev. Méd. del Hosp. General (Mexico)24, 237.

    Google Scholar 

  • Masin, M., and F. Masin, 1961: Cytophotometric data on fluorochromed cervical cells. J. Nat. Cancer Inst. (U.S.A.)27, 311.

    Google Scholar 

  • Mayor, H. D., and J. L. Melnick, 1961/62: Intracellular and extracellular reactions of viruses with vital dyes. Yale J. Biol. Med.34, 340.

    PubMed  Google Scholar 

  • Meissel, M. N., 1951: Luminescence-microscopic analysis of the functional status of the living substance (Russian). Izwest. Akad. Nauk SSSR, Ser. Fiz.15, 788.

    Google Scholar 

  • - et al., 1960: Fluorescence studies of the changes undergone by nucleoproteins and their derivatives in irradiated cells. Symposium on the Initial Effects of Ionizing Radiations on Cells, Moscow, 107.

  • - et al., 1962: Fluorescence cytology of the cancer cell. VIII Internat. Cancer Congress, Abstracts of Papers, Moscow, 137.

  • —, and A. W. Gutkina, 1953: The use of fluorescence microscopy for rapid detection of pathological changes in tissues and organs (Russian). Dokl. Akad. Nauk SSSR91, 647.

    PubMed  Google Scholar 

  • Mellors, R. C., 1956: Quantitative cytology and cytopathology: Nucleic acids and proteins in the mitotic cycle of normal and neoplastic cells. Ann. N. Y. Akad. Sci.63, art. 6, 1177.

    Google Scholar 

  • Pearse, A. G. E., 1960: Histochemistry: Theoretical and Applied. 2nd ed. New York.

  • Pirozynski, W. J., and L. von Bertalanffy, 1952: Ribonucleic acid in cytoplasm of liver cells; its localization in hyperplasia and hepatoma produced by 2-acetyl- aminofluorene. Arch. Pathology54, 450.

    Google Scholar 

  • —, 1955: Changes of cytoplasmic basophilia during carcinogenesis induced by 2-acetylaminofluorene. Exper. Med. Surg.13, 261.

    Google Scholar 

  • Raskin, J., 1960: Fluorescence microscopy in the diagnosis of cutaneous malignancy. Arch. Dermat.82, 965.

    Google Scholar 

  • Reichert, C., 1911: Das Fluoreszenzmikroskop. Physik. Z.12, 1010.

    Google Scholar 

  • Sani, G., 1961a: La microscopia di fluorescenza nella citodiagnosi oncologica. Riv. Ital. di Ginecol.45, 254.

    Google Scholar 

  • —, L. Segata, e G. Faccioli, 1961b: Sul comportamento della fluorescenza indotta mediante arancio di acridina nell'epitelio della portio e negli strisci vaginali del ratto durante il ciclo estrale. Riv. Ital. di Ginecol.45, 349.

    Google Scholar 

  • —, 1961 c: Il sistema a fluorescenza e la cito-istofluorochromizzazione con arancia di acridina. Riv. Ital. di Ginecol.45, 424.

    Google Scholar 

  • —, U. Citti, e G. Caramazza, 1961 d: La microscopia di fluorescenza nella citodiagnosi del cancro dell'utero. Bull, delle Scienze Mediche (Bologna)133, fasc.III, 1.

    Google Scholar 

  • Sani, G., 1961e: Studio istofunzionale dell'epitelio vaginale del ratto mediante fluorochromizzazione con arancio di acridina. Riv. Ital. di Ginecol.45, 437.

    Google Scholar 

  • —, U. Citti, e G. Caramazza, 1962: La fluorochromizzazione degli strisci vaginali oon arancio di acridina nella diagnosi precoce del cancro dell'utero. Minerva Ginecologica14, 389.

    PubMed  Google Scholar 

  • Schiffer, L. M., 1962: Fluorescence microscopy with acridine orange. A study of hemopoietic cells in fixed preparations. Blood19, 200.

    Google Scholar 

  • Schümmelfeder, N., 1950: Die Fluorochromierung des lebenden, überlebenden und toten Protoplasmas mit dem basischen Farbstoff Acridinorange und ihre Beziehung zur Stoffwechselaktivität der Zelle. Virchows Arch.318, 119.

    PubMed  Google Scholar 

  • —, 1956: Einfluß der Pufferlösung auf die färberische Bestimmung des Umladebereiches von Gewebselementen. Z. Zellforsch.44, 488.

    PubMed  Google Scholar 

  • - 1957: Fluoreszenzmikroskopische und cytochemische Untersuchungen über Frühschäden am Kleinhirn der Maus nach Röntgenbestrahlung. IIIe Congrès Internat, de Neuropathologie 295.

  • —, K. J. Ebschner und E. Krogh, 1957: Die Grundlage der differenten Fluorochromierung von Ribo- und Desoxyribonucleinsäure mit Acridinorange. Naturwiss.44, 467.

    Google Scholar 

  • —, R. E. Krogh und K. J. Ebschner, 1958: Färbungsanalysen zur Acridinorange-Fluorochromierung. Histochemie1, 1.

    Google Scholar 

  • Smiles, J., et al., 1957: New observations with acridine orange and primulin as vital fluorochromes. Nature (Brit.)179, 306.

    Google Scholar 

  • Starr, Th. J., et al., 1960: Cytochemical studies with psittacosis virus by fluorescence microscopy. Texas Rep. Biol. Med.18, 501.

    Google Scholar 

  • Strugger, S., 1940: Fluoreszenzmikroskopische Untersuchungen über die Aufnahme und Speicherung des Akridinorange durch lebende und tote Pflanzenzellen. Jenaische Zschr. f. Naturwiss.73, 97.

    Google Scholar 

  • - 1949: Fluoreszenzmikroskopie und Mikrobiologie. Hannover.

  • Sussman, W., 1961: Detection of gynecological cancer by fluorescence microscopy. Amer. J. Obstetr.82, 1273.

    Google Scholar 

  • Törnberg, B., B. Westin, and A. Norlander, 1960: Fluorescence microscopy and acridine-orange staining in the cytological diagnosis of atypical changes in cervical epithelium. Acta Obstetr. Gynecol. Scand.39, 517.

    Google Scholar 

  • Trentin, J. J., Y. Yabe, and G. Taylor, 1962: The quest for cancer viruses. Science137, 835.

    PubMed  Google Scholar 

  • Tweedell, K. S., 1960: Fluorescence microscopy of a frog renal adenocarcinoma. J. Morph.107, 1.

    PubMed  Google Scholar 

  • Umiker, W. O., 1961 a: Cytologic diagnosis of carcinoma of the urinary bladder by fluorescence microscopy. Acta Cytol.5, 245.

    PubMed  Google Scholar 

  • —, 1961 b: Fluorescence microscopy in exfoliative cytology of oral carcinomas. Oral Surg., Oral Med., and Oral Path.14, 1269.

    Google Scholar 

  • —, and L. Pickle, 1960: The cytologic diagnosis of lung cancer by fluorescence microscopy. Lab. Investigation9, 613.

    Google Scholar 

  • Vassar, P. S., and C. F. A. Culling, 1959: Fluorescent stains, with special reference to amyloid and connective tissues. Arch. Pathology68, 487.

    Google Scholar 

  • Vinegar, R., 1956: Metachromatic differential fluorochroming of living and dead ascites tumor cells with acridine orange. Cancer Res.16, 900.

    PubMed  Google Scholar 

  • Vita, D. G. de, y H. S. Correa, 1961: Citodiagnóstico das células neoplásticas pela fluorescência. Rev. Brasil. Cirurgia42, 119.

    Google Scholar 

  • Weiss, P., 1962: Motion pictures show nerve fibre as stream. Report. Med. Tribune,May 28, 21.

    Google Scholar 

  • Weiss, P., and H. B. Hiscoe, 1948: Experiments on the mechanism of nerve growth. J. Exper. Zool.107, 315.

    Google Scholar 

  • Weissmann, Ch., 1953: Die Vitalfärbung mit Acridinorange an Amphibienlarven. Z. Zellforsch.38, 374.

    PubMed  Google Scholar 

  • —, und A. Gilgen, 1956: Die Fluorochromierung lebender Ehrlich-Ascites-Carcinomzellen mit Acridinorange und der Einfluß der Glycolyse auf das Verhalten der Zelle. Z. Zellforsch.44, 292.

    PubMed  Google Scholar 

  • Wellmann, K. F., 1962: Fluorescence microscopic study of normal and diseased squamous epithelium in tissue sections from the uterine cervix. Surg., Gyn., Obstetr.114, 617.

    Google Scholar 

  • Williams, M. G., 1961: Histochemical observations ofVerruca vulgaris. J. Investigat. Derm.37, 279.

    Google Scholar 

  • Wittekind, D., 1958a: Die Vitalfärbung des Mäuseascites-Carzinoms mit Acridinorange. Z. Zellforsch.49, 58.

    PubMed  Google Scholar 

  • —, 1958b: Beobachtungen über eine besondere Art der Acridinorangespeicherung in vitalen Ergußzellen. Dtsch. Arch. f. Klin. Med.205, 411.

    Google Scholar 

  • —, 1958c: Bemerkungen zu dem Einfluß der Fixation auf die Fluorochromierung mit Acridinorange. Acta Histochem., Suppl.I, 247.

    Google Scholar 

  • —, 1959: Über die derzeitige Bedeutung der Vitalfärbung, speziell der Vitalfluorochromierung in der Cytologie, und über Möglichkeiten ihrer weiteren Anwendung, insbesondere in Kombination mit anderen Methoden. Mikroskopie14, 9.

    PubMed  Google Scholar 

  • —, 1960: Über Entstehung, Morphologie und gegenseitige Beziehungen intraplasmatischer Vacuolenbildungen in lebenden Tumorzellen aus Ergüssen seröser Höhlen. Virchows Arch.333, 311.

    Google Scholar 

  • —, und A. Völcker, 1958: Untersuchungen an Ergüssen seröser Höhlen mit dem kombinierten Phasenkontrast-Fluoreszenzmikroskop. Z. Klin. Med.155, 403.

    PubMed  Google Scholar 

  • Wolf, M. K., and S. B. Aronson, 1961: Growth, fluorescence and metachromasy of cells cultured in the presence of acridine orange. J. Histochem. a. Cytochem.9, 22.

    Google Scholar 

  • Young, M. R., 1961: Principles and technique of fluorescence microscopy. Quart. J. Microscop. Sci.102, 419.

    Google Scholar 

  • Zanella, E., e L. Chiampo, 1961: Un metodo rapido per la diagnostica citologica intraoperatoria mediante la microscopia a fluorescenza. Boll. soc. ital. biol. sper. (Naples)37, 652.

    Google Scholar 

  • Zeiger, K., und H. Harders, 1951: Über vitale Fluorochromfärbung des Nervengewebes. Z. Zellforsch.36, 62.

    PubMed  Google Scholar 

  • —, und W. Schmidt, 1957: Über die Natur der bei Intoxikation mit Acridinorange in tierischen Zellen entstehenden Stoffablagerungen. Z. Zellforsch.45, 578.

    PubMed  Google Scholar 

  • —, und M. Wiede, 1954: Die Speicherung von Acridinorange in der Froschleber und ihr Einfluß auf das Ausscheidungsvermögen der Leberzelle. Z. Zellforsch.40, 401.

    PubMed  Google Scholar 

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  1. Department of Zoology, University of Alberta, Edmonton, Canada

    Ludwig von Bertalanffy

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von Bertalanffy, L. Acridine orange fluorescence in cell physiology, cytochemistry and medicine. Protoplasma 57, 51–83 (1963). https://doi.org/10.1007/BF01252046

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