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Kernkontrollierte Lactatdehydrogenase inAcetabularia

Nucleus dependent lactic dehydrogenase inAcetabularia

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Zusammenfassung

In kernhaltigen, aber auch in kernlosen Zellen vonAcetabularia wird wÄhrend des Wachstums die LDH-AktivitÄt bis zum Beginn der Hutbildung vermehrt. Verschiedene Arten vonAcetabularia weisen bezüglich der LDH entweder durch Zahl der Banden oder Position der Banden Unterschiede voneinander auf. Heterologe Transplantate bzw. Implantate lassen einige Wochen nach der Operation erkennen, da\ die artspezifischen Isozymbanden des Cytoplasmas verschwinden und die vom Kern determinierten erscheinen. Zumindest ein Teil der LDH-AktivitÄt ist an die Chloroplasten gebunden. Dieses wurde mit Hilfe der Saccharose-Dichtegradienten-Zentrifugation und auf histochemischem Wege nachgewiesen.

Summary

In nucleate as well as in anucleate cells ofAcetabularia LDH activity is increased until the cap is formed. Different species ofAcetabularia can be distinguished by the electrophoretic behavior of the LDH. Heterologous grafts of nucleus containing rhizoids or of isolated nuclei to anucleate cytoplasm result in the disappearance of the LDH pattern of the cytoplasm and in the appearance of a new one which is specific for the species of the nucleus. At least part of the enzyme activity is linked to the chloroplasts as was shown by sucrose density gradient centrifugation and by histochemical techniques.

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Author notes

  1. W. Reuter & H. G. Schweiger

    Present address: Max-Planck-Institut für Zellbiologie, Wilhelmshaven, BRD

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    1. W. Reuter
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    2. H. G. Schweiger
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    Additional information

    Wir danken Herrn Dr. G.Werz für Anregungen beim histochemischen Nachweis und Herren H.Kretschmer für seine Hilfe bei der PrÄparation der Transplantate und Implantate.

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    Reuter, W., Schweiger, H.G. Kernkontrollierte Lactatdehydrogenase inAcetabularia . Protoplasma 68, 357–368 (1969). https://doi.org/10.1007/BF01251620

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