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A rapid radioimmunoassay method for the detection of hepatitis B antigen

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Summary

An increase in incubation temperature under humid conditions, and a decrease in an incubation time was used to shorten the radioimmunoassay (RIA) method for hepatitis B antigen (HB-Ag) assay. Incubation of the serum to be tested at 47° C for 120 minutes for primary adsorption was as sensitive as the regular test at room temperature (RT°) for 16 hours. In addition, the washing procedure for the removal of labelled antibody was shortened. In order to break hepatitis B antigenantibody (HB-Ag-Ab) complexes before testing the HB-Ag, sera were heated at 56° C for 30 minutes and then retested by RIA. The heating of the sera resulted in a 2 per cent higher yield of HB-Ag positives. Therefore, we recommend that sera be heated before testing for HB-Ag.

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Author notes

  1. G. M. Kouroupis

    Present address: Department of Microbiology of the Wellesley and Princess Margaret Hospitals, Canada

Authors and Affiliations

  1. Department of Microbiology of the Wellesley and Princess Margaret Hospitals, Canada

    W. -D. Leers

  2. Department of Medical Microbiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

    G. M. Kouroupis & W. -D. Leers

Authors
  1. G. M. Kouroupis
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  2. W. -D. Leers
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Kouroupis, G.M., Leers, W.D. A rapid radioimmunoassay method for the detection of hepatitis B antigen. Archiv f Virusforschung 44, 370–376 (1974). https://doi.org/10.1007/BF01251018

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  • DOI: https://doi.org/10.1007/BF01251018

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