Summary
A virus (HSIS) originating from dead owls was successfully cultivated in chicken embryo fibroblasts. Its replication was inhibited by IUDR. Tissue cultured virus proved sensitive to ether, chloroform, 0.5 per cent trypsin, and to pH levels of 4.0 or lower. Infectivity was rapidly destroyed at 56° C. Negatively stained naked virions of 100 nm average diameter were seen, and enveloped virions with 160–250 nm size. The capsid was built up of hollow cyclindrical capsomeres, arranged in equilateral triangles, carrying 5 capsomeres along each edge. Cubical symmetry and icosahedron structure yielded a total number of 162 capsomeres. All these biochemical and biophysical data lead to classification of HSIS virus into the genus herpesvirus. Biological properties described in a foregoing paper sustained such grouping, and indicated that the agent was a new avian herpesvirus for which the nameherpesvirus strigis was proposed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Andrewes, Chr., andD. M. Horstman: The susceptibility of viruses to ethyl ether. J. gen. Microbiol.3, 290–297 (1949).
Andrewes, Chr., andH. G. Pereira: In: Viruses of Vertebrates. 2nd Ed., p. 432. London: Baillière, Tindall and Cassell, 1967.
Almeida, J., A. F. Howatson, andM. G. Williams: Morphology of varicella (chicken pox) virus. Virology16, 353–355 (1962).
Bögel, K., undH. Böhm: Ein Rhinovirus des Rindes. Zbl. Bakt. I. Abt. Orig.187, 2–14 (1962).
Bögel, K., undA. Mayr: Untersuchungen über die Chloroformresistenz der Enteroviren des Rindes und des Schweines. Zbl. Vet.-Med.8, 908–922 (1961).
Bürki, F.: Studien über bovine Enteroviren. 1. Mitteilung. Zbl. Vet.-Med.9, 748 to 759 (1962).
Bürki, F., H. Burtscher, andM. Sibalin: Herpesvirus strigis, a new avian species of the genus herpesvirus. I. Biological properties. Arch. ges. Virusforsch.43, 14–24 (1973).
Bürki, F., andL. Pichler: Further biochemical testing of feline picornaviruses. Arch. ges. Virusforsch.33, 126–133 (1971).
Burtscher, H.: Die virusbedingte Hepatosplenitis infectiosa strigum. 1. Mitteilung: Morphologische Untersuchungen. Path. Vet.2, 227–255 (1965).
Burtscher, H., undA. Schumacher: Morphologische Untersuchungen zur Virus-ätiologie der Hepatosplenitis infectiosa strigum. Path. Vet.3, 506–528 (1966).
Caspar, D. L. D., andA. Klug: Physical principles in the construction of regular viruses. Cold Spr. Harb. Symp. quant. Biol.27, 1–24 (1962).
Cruickshank, J. G., D. M. Berry, andB. Hay: The fine structure of infectious laryngotracheitis virus. Virology20, 376–378 (1963).
Dinter, Z.: Relationship between bovine virus diarrhea virus and hog cholera virus. Zbl. Bakt. I. Abt. Orig.188, 475–486 (1963).
Horne, R. W., andP. Wildy: Symmetry in virus architecture. Virology15, 348 to 373 (1961).
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physics32, 1637 (1961).
Reed, L. J., andH. A. Muench: A simple method of estimating fifty percent endpoints. Amer. J. Hyg.27, 493–497 (1938).
Reissig, M., andA. S. Kaplan: The morphology of noninfective pseudorabies virus produced by cells treated with 5-fluorouracil. Virology16, 1–8 (1962).
Schettler, C. H.:In vitro Untersuchungen über die Eigenschaften des Virus der Hepatitis et Splenitis Infectiosa Strigum. In: Erkrankungen der Zootiere, pp. 205 to 209. Berlin: Akademie Verlag, 1970.
Schneweis, K. E.: Über die Haltbarkeit von drei Herpes simplex Virusstämmen bei tiefen Temperaturen. Z. Hyg. Infect.-Kr.147, 319–326 (1961).
Watrach, A. M., L. E. Hanson, andM. A. Watrach: The structure of infectious laryngotracheitis virus. Virology21, 601–608 (1963).
Wildy, P.: Classification and nomenclature of viruses. Monographs in Virol. Vol. 5, p. 81. Basel-München-Paris-London-New York-Sidney: S. Karger, 1971.
Wildy, P., W. C. Russell, andR. W. Horne: The morphology of herpesvirus. Virology12, 204–222 (1960).
Zwillenberg, L. O., andF. Bürki: On the capsid structure of small feline and bovine RNA viruses. Arch, ges. Virusforsch.19, 373–384 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sibalin, M., Gerstl, F., Pichler, L. et al. Herpesvirus strigis, a new avian herpesvirus. Archiv f Virusforschung 44, 361–369 (1974). https://doi.org/10.1007/BF01251017
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01251017