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Propagation and plaquing of Dugbe virus (an ungrouped Nigerian arbovirus) in various mammalian and arthropod cell lines

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Summary

Dugbe virus (tick-borne) multiplied to high titres in PS, BSC-1 LLC-MK2, BHK-2 and L cells.Boophilus microplus (tick) cells also supported replication; but no growth in eitherAedes albopictus orA. aegypti (mosquito) cells. Plaques were also produced in all the mammalian cells with the exception of LLC-MK2. A regular feature of the cytopathology in the mammalian cells was the formation of basophilic, pyroninophilic, cytoplasmic inclusion bodies, which by immunofluorescent staining were shown to be depots of viral antigen concentration.

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Author notes

  1. Tam S. David-West

    Present address: Virus Research Laboratory, Faculty of Medicine, University of Ibadan, Ibadan, Nigeria

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    1. Tam S. David-West
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    Most of this work was done at the Virology Division, National Institute for Medical Research, Mill Hill, London, England, and supported by a Commonwealth Tropical Medicine Research Fellowship.

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    David-West, T.S. Propagation and plaquing of Dugbe virus (an ungrouped Nigerian arbovirus) in various mammalian and arthropod cell lines. Archiv f Virusforschung 44, 330–336 (1974). https://doi.org/10.1007/BF01251014

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    • DOI: https://doi.org/10.1007/BF01251014

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