Summary
This study suggests that fluorescent microscopy may be feasible for the “bedside” diagnosis of respiratory diseases caused by adenoviruses and mycoplasma. Using throat washings or nasopharyngeal scrapings with serologic tests for confirmation, it was determined that approximately 50% of the adenovirus and 73% of the mycoplasma infections could be rapidly detected on a routine basis. It is assumed that a greater correlation could be obtained with the institution of better patient specimen collection.
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References
Aktenstein, M. S., andO. W. Dandridge: A new serologic test for adeno-virus infection. J. Immunol.100, 831–834 (1968).
Brown, G. C., H. F. Maassab, J. A. Veronelli, andT. Francis, Jr.: Rubella antibodies in human serum: Detection by indirect fluorescent antibody technique. Science145, 943–945 (1964).
Hatch, H. H., S. S. Kalter, andJ. W. Ajello: Identification of poliovirus isolates with fluorescent antibody. Proc. Soc. exp. Biol. (N.Y.)107, 1–4 (1961).
Hers, J. F.: Fluorescent antibody technique in respiratory viral diseases, in Conference on Newer Respiratory Diseases. Amer. Rev. resp. Dis., pp. 316–333 (1962).
Kalter, S. S.: Procedures for Routine Laboratory Diagnosis of Virus and Rickettsial Disease, Minneapolis, Minn.: Burgess Publishing Co. (1963).
Kalter, S. S., M. H.Hatch, and J. W.Ajello: The laboratory diagnosis of poliomyelitis with fluorescent antibodies. Bact. Proc.89, (1959).
Liu, C.: Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody. Proc. Soc. exp. Biol. (N.Y.)92, 883–887 (1956).
Liu, C.: Fluorescent antibody techniques, in Diagnostic Procedure for Viral and Rickettsial Disease, 3rd. ed., New York, N.Y. (E. H. Lennette andN. J. Schmidt, ed.). Amer. publ. Hlth Ass., Inc., p. 177 (1964).
Llanes-Rodas, R., andC. Liu: Rapid diagnosis of measles from urinary sediments stained with fluorescent antibody. New England J. Med.275, 516–523 (1966).
McQuillen, J., andP. S. Gardner: Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques. Brit. Med. J.1, 602–605 (1968).
Mogabgab, W. J.: Mycoplasma pneumoniae and adenovirus respiratory illness in military and university personnel, 1959–1966. Amer. Rev. resp. Dis.97, 345–358 (1968).
Rapp, F., and I.Gordon: Development and spread of measles virus infection in human cells. Bact. Proc.58 (1958).
Reinarz, J. A., E. R.Rhoades, V.Armour, and S. S.Kalter: Etiology of non-bacterial pneumonia: A prospective and continuing study. AMLC-TR-68-11 August, 1968 (in press).
Shaw, E. D., A. Newton, A. W. Powell, andC. J. Friday: Fluorescent antigen-antibody reactions in Coxsackie and ECHO enteroviruses. Virology15, 208–210 (1961).
Tateno, I., M. Makino, andY. Takeuchi: Rapid diagnosis of influenza B with fluorescent antibody. Jap. J. exp. Med.36, 301–320 (1966).
Tateno, I., S. Suyuki, S. Nakamura, O. Kitamoto, A. Kawamura, Jr.,M. Makino, U. Takeuchi, andT. Sonoguchi: Rapid diagnosis of influenza by means of fluorescent antibody technic. I. Some basic informations. II. Relation between immunologic, serologic and clinical findings. III. Some new aspects of influenza virus infection revealed by fluorescent antibody technic. Jap. J. exp. Med.35, 383–417 (1965).
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Kalter, S.S., Armour, V. & Reinarz, J.A. Rapid diagnosis of respiratory disease due to adenovirus and mycoplasma. Archiv f Virusforschung 28, 34–40 (1969). https://doi.org/10.1007/BF01250843
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DOI: https://doi.org/10.1007/BF01250843