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Die Gewinnung unterschiedlicher Hämagglutinin-fraktionen aus Influenzaviren mit Hilfe von DEAE-Zellulose-Ionenaustauscher

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Zusammenfassung

In den vorliegenden Untersuchungen wurde versucht, die durch Äthereinwirkung auf das Influenzavirus A2/Sing./1/57 entstehenden Hämagglutininfraktionen an Austauschersäulen (DEAE-Zellulosesäulen) zu trennen.

Die Ergebnisse sprechen dafür, daß ätherdestruiertes Influenzavirusmaterial bei der Elution von DEAE-Zellulosesäulen unterschiedliche Hämagglutininfraktionen ergibt.

Unserer med.-technischen Assistentin FrauIrmgard Braun danken wir für ihre sorgfältige Mitarbeit.

Summary

In the present investigations, the attempt was made to separate the haemagglutinin fractions resulting from the action of ether on the influenza virus A2/Sing./1/57, on exchange colums (DEAE cellulose colums).

The results suggest that influenza virus material destroyed by ether gives distinct haemagglutinin fractions on elution from DEAE cellulose colums.

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Literatur

  1. Brand, G., H. Lippelt undF. Müller: Untersuchungen über den Einfluß der Blutkörperchen auf den Hämagglutinationstest mit Influenza- und Mumpsvirus. Z. Hyg. Infekt.-Kr.139, 293 (1954).

    Google Scholar 

  2. Brand, G.: Zur Antigen-struktur des Influenzavirus. Habilitationsschrift, West-Berlin 1956.

    Google Scholar 

  3. Fabiyi, A., F. S. Lief, andW. Henle: Antigenic analysis of influenza viruses by complement fixation. II. The production of antisera to strain- specific V antigens in guinea pigs. J. Immunol.81, 467 (1958).

    PubMed  Google Scholar 

  4. Flick, J. A.: Use of formalin-treated red cells for the study of influenza virus hemagglutinating activity. Proc. Soc. exp. Biol. (N. Y.)68, 448 (1948).

    Google Scholar 

  5. Frisch-Niggemeyer, W. andL. Hoyle: The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether desintegration. J. Hyg. (Cambridge)54, 201 (1956).

    Google Scholar 

  6. Frisch-Niggemeyer, W.: The internal structure of influenza virus. Z. Naturforschg.14b, 168 (1959).

    Google Scholar 

  7. Hoyle, L.: The multiplication of influenza viruses in the fertile egg. J. Hyg. (Cambridge)48, 277 (1950).

    Google Scholar 

  8. Hoyle, L.: Structure of influenza virus. The relation between biological activity and chemical structure of virus fractions. J. Hyg. (Cambridge)50, 229 (1952).

    Google Scholar 

  9. Hoyle, L., B. Jolles, andR. G. Mitchell: The incorporation of radioactive phosphorus in the influenza virus and its distribution in serologically active virus fractions. J. Hyg. (Cambridge)52, 119 (1954).

    Google Scholar 

  10. Hoyle, L. andN. B. Finter: The use of influenza virus labelled with radiosulphur in studies of the early stages of the interaction of virus with the host cell. J. Hyg. (Cambridge)55, 290 (1957).

    Google Scholar 

  11. Klemperer, H. G. andH. G. Pereira: Study of adenovirus antigens fractionated bychromatography on DEAE-cellulose. Virology9, 536 (1959).

    PubMed  Google Scholar 

  12. Lief, F. S., A. Fabiyi, andW. Henle: Antigenic analysis of influenza viruses by complement fixation. I. The production of antibodies to the soluble antigen in guinea pigs. J. Immunol.80, 53 (1958).

    PubMed  Google Scholar 

  13. Lief, F. S., M. Ostapiak, A. Fabiyi, andW. Henle: Antigenic analysis of influenza viruses by complement fixation. III. Rapid identification of new isolates. J. Immunol.81, 478 (1958).

    PubMed  Google Scholar 

  14. McCrea, J. F.: persönliche Mitteilung vom 18. 1. 1960.

  15. MeCrea, J. F. andJ. O'Loughlin: Separation of vaccinia hemagglutinin from infectious virus particles by chromatography on DEAE colums. Virology8, 127 (1959).

    PubMed  Google Scholar 

  16. Peterson, E. A. andH. A. Sober: Chromatography of proteins. I. Cellulose ion-exchange adsorbents. J. Am. Chem. Soc.78, 751 (1956).

    Google Scholar 

  17. Prager, M. D. andR. J. Speer: Chromatography of McCaslan pole bean hemagglutinin on DEAE-cellulose. Proc. Soc. exp. Biol. a. Med.100, 68 (1959).

    Google Scholar 

  18. Salk, J. E.: A simplified procedure for titrating hemagglutinating capacity of influenza-virus and the corresponding antibody. J. Immunol.49, 87 (1944).

    Google Scholar 

  19. Sober, H. A. andE. A. Peterson: Chromatography of proteins on cellulose ion-exchangers. J. Am. Chem. Soc.76, 1711 (1954).

    Google Scholar 

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Author notes

  1. Bernhard Schmidt Dipl.-Chem., Dieter Hartmann & Klaus Groβgebauer Dr. med.

    Present address: Institut für Hygiene und Medizinische Mikrobiologie, FreienUniversität Berlin, Deutschland

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    1. Bernhard Schmidt Dipl.-Chem.
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    2. Dieter Hartmann
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    3. Klaus Groβgebauer Dr. med.
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    Schmidt, B., Hartmann, D. & Groβgebauer, K. Die Gewinnung unterschiedlicher Hämagglutinin-fraktionen aus Influenzaviren mit Hilfe von DEAE-Zellulose-Ionenaustauscher. Archiv f Virusforschung 10, 361–367 (1960). https://doi.org/10.1007/BF01250681

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    • DOI: https://doi.org/10.1007/BF01250681

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