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Delay of the appearance of cytopathic effect by latent viruses in monkey kidney cell cultures after freezing and storing at low temperature

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Summary

Monkey kidney cell suspensions were frozen and stored at low temperature for as long as 8 months. Cells were frozen as fresh trypsinized cells and after a primary culturein vitro. Cells recovered after freezing were cultured as primary and secondary cultures to check for spontaneous contaminants. Cell-freezing process showed to delay the appearance of spontaneous lesions caused by adventitious agents in monkey kidney cells. This delay was less important if frozen cells were stored at low temperature for as long as 8 months. Cell-freezing showed to be a good method for finding the presence of spontaneous contaminants in the eultured cells and may help to select monkey kidney cell suspensions in vaccine production or any other experimental works.

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References

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Author notes

  1. A. Chagnon

    Present address: Institut de Microbiologie et d'Hygiène de l'Université de Montréal, Canada

Authors and Affiliations

  1. Institut de Microbiologie et d'Hygiène de l'Université de Montréal, Canada

    V. Pavilanis

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  1. A. Chagnon
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  2. V. Pavilanis
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Additional information

This work was supported by a grant from the Ministry of Health of the Province of Quebec (Federal-Provincial Public Health Grant).

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Chagnon, A., Pavilanis, V. Delay of the appearance of cytopathic effect by latent viruses in monkey kidney cell cultures after freezing and storing at low temperature. Archiv f Virusforschung 19, 435–441 (1966). https://doi.org/10.1007/BF01250611

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  • DOI: https://doi.org/10.1007/BF01250611

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