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Fractionation of respiratory syncytial (RS) virus components by centrifugation techniques

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Summary

After equilibrium centrifugation in CsCl gradients of concentrated RS virus material, infectious particles were recovered in fractions with densities varying between 1.21 to 1.23 g/cc., whereas CF antigen activity mainly accumulated in the density range 1.25 to 1.34 g/cc. Small quantities of CF antigens also appeared at lower densities. Treatment with Tween 80 and ether eliminated all infectivity without a concomitant destruction of CF antigen. The buoyant density distribution of CF activity in such a preparation was identical with that of untreated material except that all low density (1.25 g/cc.) activity had disappeared.

Centrifugation of virus material layered on top of a discontinuous CsCl gradient revealed the presence of two populations of particles. One represented rapidly sedimenting particles carrying all infectivity and about 10% of the total CF antigen activity. The other carried all of the remaining CF antigen activity and had the character of a soluble antigen.

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Author notes

  1. B. Bloth

    Present address: Department of Virus Research, Karolinska Institute, School of Medicine, Stockholm, Sweden

Authors and Affiliations

  1. Department of Virus Research, Karolinska Institute, School of Medicine, Stockholm, Sweden

    E. Norrby

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  1. B. Bloth
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  2. E. Norrby
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Supported by grants from the Swedish Medical Research Council (Project No. 16X-748-01).

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Bloth, B., Norrby, E. Fractionation of respiratory syncytial (RS) virus components by centrifugation techniques. Archiv f Virusforschung 19, 385–392 (1966). https://doi.org/10.1007/BF01250607

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  • DOI: https://doi.org/10.1007/BF01250607

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