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No alterations in the 5-HT1A-mediated inhibition of forskolin-stimulated adenylate cyclase activity in the hippocampal membranes from rats chronically treated with lithium or antidepressants

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Summary

In order to determine the relevance of 5-HT1A-related signal transduction in the mode of action of lithium and antidepressants, the effects of long-term treatment with these drugs on the 5-HT1A-mediated inhibition of forskolin-stimulated adenylate cyclase activity were investigated in the rat hippocampal membranes. Chronic administration of antidepressants altered neither the [3H]8-hydroxy-2-(di-n-propylamino)tetralin ([3H]8-OH-DPAT) binding sites nor the inhibition of forskolin-stimulated adenylate cyclase activity by 5-HT. Long-term treatment with lithium did not affect the inhibitory effect of 5-HT on the forskolin-stimulated adenylate cyclase activity, either. Neither the stimulation by forskolin nor the inhibition by guanyl-5′-ylimidodiphosphate (Gpp(NH)p) of adenylate cyclase activity was not influenced by lithium treatment, suggesting that lithium has no effects on the components of adenylate cyclase system distal to the 5-HT1A receptors.

These results indicate that the 5-HT1A-mediated neural transmission has not such an important relevance in the mechanisms of action of lithium or antidepressants.

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  1. Y. Odagaki

    Present address: Department of Psychiatry and Neurology, Hokkaido University School of Medicine, Sapporo, Japan

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  1. Department of Psychiatry and Neurology, Hokkaido University School of Medicine, Sapporo, Japan

    T. Koyama & I. Yamashita

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  1. Y. Odagaki
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  2. T. Koyama
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  3. I. Yamashita
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Odagaki, Y., Koyama, T. & Yamashita, I. No alterations in the 5-HT1A-mediated inhibition of forskolin-stimulated adenylate cyclase activity in the hippocampal membranes from rats chronically treated with lithium or antidepressants. J. Neural Transmission 86, 85–96 (1991). https://doi.org/10.1007/BF01250570

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  • DOI: https://doi.org/10.1007/BF01250570

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