Summary
Eleven lines and sublines of larynx carcinoma (HEp-2), cervical cancer (HeLa) and dedifferentiated astrocytoma (DAPT) cells were examined and viruses were isolated from eight lines. Electronmicroscopic, biophysical and biochemical examinations of the viruses showed that they belong to the B-type oncornaviruses. The virions had a size of 100 to 150 nm, they banded at densities of 1.16 to 1.17 g/ ml in sucrose gradients and RNA from the virions contained 60–70S component. The reproduction of the viruses was inhibited by actinomycin D, and the virions possessed reverse transcriptase (RNA-dependent DNA polymerase) activity. The isolated viruses differed antigenically from mouse mammary tumor virus.
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Bykovsky, A.F., Miller, G.G., Yershov, F.I. et al. B-type oncornaviruses isolated from continuous human cancer cell Lines. Archiv f Virusforschung 42, 21–35 (1973). https://doi.org/10.1007/BF01250504
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DOI: https://doi.org/10.1007/BF01250504