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Studies with a rhinovirus of bovine origin

II. Some physical and chemical properties of strain RS 3x

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Summary

A bovine rhinovirus (strain RS 3x) was fo und to be most stable at pH 7.0, but was rapidly inactivated at pH 5 and pH 10.5. The virus was approximately 25 nm in diameter by filtration, and possessed a hydrated density of approximately 1.40 g/ml in caesium chloride. Replication was not inhibited by the presence of 5-iodo-2′-deoxyuridine.

The virus was rapidly inactivated by trypsin and sodium dodecyl sulphate but was unaffected by chloroform or sodium desoxycholate. Infectivity was rapidly lost at 37δC, and very rapidly at 50°C, at which temperature no stabilisation occurred in the presence of molar magnesium chloride.

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Author notes

  1. P. R. Ide

    Present address: Health of Animals Branch, Animal Pathology Division, Canada

  2. J. H. Darbyshire

    Present address: The Animal Virus Research Institute, Pirbright, Woking, Surrey, England

Authors and Affiliations

  1. Central Veterinary Laboratory, Weybridge, Surrey, UK

    P. R. Ide

  2. Department of Agriculture, Atlantic Area Branch Laboratory, Sackville, New Brunswick, Canada

    J. H. Darbyshire

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  1. P. R. Ide
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Ide, P.R., Darbyshire, J.H. Studies with a rhinovirus of bovine origin. Archiv f Virusforschung 36, 177–188 (1972). https://doi.org/10.1007/BF01250308

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  • DOI: https://doi.org/10.1007/BF01250308

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