Summary
Studies were made on the growthin vitro of a strain (RS 3x) of a bovine rhinovirus using several cell systems. The cytopathic effect in calf kidney (CK) cells occurred more rapidly when a medium containing low levels of sodium bicarbonate was employed, and this was more progressive in cultures incubated while being rolled than in those incubated in a stationary position. Infectivity titres attained by the virus in CK cells were initially about equal at both 33°C and 37°C, but higher and more persistent titres were subsequently obtained at 33° C. Cultures grown in the presence of bovine serum containing a neutralizing titre of 1:2000 or greater to the RS 3x strain were markedly inhibitory to virus growth, even when the cells were washed prior to inoculation and subsequently maintained in a serum-free medium. It was found necessary to change the culture medium to one which contained foetal calf serum, free of specific antibody, 3 days prior to infection with virus and to wash the cells at least 5 times before use. The RS 3x strain replicated in monolayer cultures of bovine origin as well as in the differentiated cells of calf nasal turbinate and tracheal organ cultures.
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Ide, P.R., Darbyshire, J.H. Studies with a rhinovirus of bovine origin. Archiv f Virusforschung 36, 166–176 (1972). https://doi.org/10.1007/BF01250307
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DOI: https://doi.org/10.1007/BF01250307