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Vaccinia virus: Kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin

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Summary

The optimum temperature and time of incubation of the vaccinia hemagglutination-inhibition test were 37°C and 16 hours, respectively. A convenient method for preparing vaccinia hemagglutinating antigen consisted of infecting one-day-old primary chick embryo fibroblasts with a high multiplicity of vaccinia virus, employing a maintenance medium devoid of serum, preparing a 20-fold concentration of virus-infected cells, and finally extracting the concentrated antigen homogenate with genetron.

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Author notes

  1. Thomas C. O'brien

    Present address: U.S. Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, Division of Biologies Standards, Laboratory of Virology and Rickettsiology, Bethesda, Maryland, USA

Authors and Affiliations

  1. U.S. Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, Division of Biologies Standards, Laboratory of Virology and Rickettsiology, Bethesda, Maryland, USA

    Nicola M. Taurkaso

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O'brien, T.C., Taurkaso, N.M. Vaccinia virus: Kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin. Archiv f Virusforschung 36, 158–165 (1972). https://doi.org/10.1007/BF01250306

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