Summary
A cytopathogenic agent, designated CHV-BR, isolated from genital lesions in dogs has been shown to be a herpesvirus. It contained DNA, was ether-and heat-sensitive, and was unstable at low pH. Electron micrographs revealed a virion of typical herpesvirus structure. The CHV-BR isolate produced a marked cytopathic effect (CPE) in dog kidney cell cultures but failed to grow in rabbit kidney and human cell lines. The CPE in dog kidney cells consisted of extensive polykaryocytosis, and the formation of intranuclear type-A inclusion bodies. The emergence of a non-polykaryocytogenic CPE variant during serial passage of the isolate in dog cells was described. Neutralization and immunofluorescence tests showed that CHV-BR was serologically similar to the F-205 strain of canine herpesvirus (CHV) but was not related to other human and animal herpesviruses. Differences between the CHV-BR isolate and the CHV strains isolated elsewhere were discussed.
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Andrewes, C. H., andD. M. Horstmann: The susceptibility of viruses to ethyl ether. J. gen. Microbiol.3, 290 (1949).
Appel, M. J. G., M. Menegus, I. M. Parsonson, andL. E. Carmichael: Pathogenesis of canine herpesvirus in specific-pathogen-free dogs: 5-to 12-week old pups. Amer. J. vet. Res.30, 2067 (1969).
Binn, L. N., G. A. Eddy, E. C. Lazae, J. Helms, andT. Murnana: Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. exp. Biol. (N.Y.)126, 140 (1967).
Binn, L. N., W. P. Koughan, andE. Lazar: A simple plaque procedure for comparing antigenic relationships of canine herpesvirus. J. Amer. vet. med. Ass.156, 1724 (1970).
Carmichael, L. E., J. D. Strandberg, andF. D. Barnes: Identification of a cytopathogenic agent infectious for puppies as a canine herpesvirus. Proc. Soc. exp. Biol. (N.Y.)120, 644 (1965).
Cornwell, H. J. C., andN. G. Wright: Neonatal canine herpesvirus infection: A review of present knowledge. Vet. Rec.84, 2 (1969).
Davenport, H. A.: Histological and Histochemical Technics p. 372. Philadelphia: W. B. Saunders, 1961.
Deeley, E. M.: An integrating microdensitometer for biological cells. J. sci. Instrum.32, 263 (1955).
Falke, D.: Isolation of two variants with different cytopathic properties from a strain of herpes B virus. Virology14, 492 (1961).
Jacobson, W., andM. Webb: Two kinds of nucleic acid. Exp. Cell Res.3, 163 (1952).
Kakuk, T. J., G. H. Conner, R. F. Langham, J. A. Moore, andJ. R. Mitchell: Isolation of a canine herpesvirus from a dog with malignant lymphoma. Amer. J. vet. Res.30, 1951 (1969).
Kaplan, A.: Herpes simplex and pseudorabies viruses. Virology Monographs No. 5. Wien and New York: Springer-Verlag, 1969.
Karpas, A., F. G. Garcia, F. Calvo, andR. E. Cross: Experimental production of canine tracheobronchitis with canine herpesvirus isolated from naturally infected dogs. Amer. J. vet. Res.29, 1251 (1968).
Kleger, B., andJ. E. Prier: Herpes simplex infection of the female genital tract. II. Association of cytopathic effect in cell culture with site of infection. J. infect. Dis.120, 376 (1969).
Madin, S. H., andN. B. Darby: Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. exp. Biol. (N.Y.)98, 574 (1958).
Mayor, H. D., andR. R. Diwan: Studies on the acridine orange staining of two purified RNA viruses: poliovirus and tobacco mosaic virus. Virology14, 74 (1961).
Motohashi, T., andM. Tajima: Isolation of a canine herpesvirus from a diseased adult dog in Japan. Jap. J. vet. Sci.28, 307 (1966).
Munk, K., undD. Donner: Cytopathischer Effekt und Plaque-Morphologie verschiedener Herpes-Simplex-Virus-Stämme. Arch. ges. Virusforsch.13, 529 (1963).
Nahmias, A. J., andW. R. Dowdle: Antigenic and biologic differences in herpesvirus hominis. Progr. med. Virol.10, 110 (1968).
Nii, S., andY. Maeda: Studies of herpes zoster virusin vitro. I. Isolation of two variants. Biken J.12, 219 (1969).
Poste, G.: Virus-induced polykaryocytosis and the mechanism of cell fusion. Advanc. Virus Res.16, 303 (1970).
Poste, G., andN. King: Isolation of a herpesvirus from the canine genital tract: association with infertility, abortion and stillbirths. Vet. Rec.88, 229 (1971).
Reed, L. J., andH. Muench: A simple method of estimating fifty percent end points. Amer. J. Hyg.27, 493 (1938).
Reissig, M., D. W. Howes, andJ. L. Melnick: Sequence of morphological changes in epithelial cell cultures infected with poliovirus. J. exp. Med.104, 289 (1956).
Spertzel, R. O., D. L. Huxsoll, S. J. McConnell, L. N. Binn, andR. H. Yager: Recovery and characterization of a herpes-like virus from dog kidney cell cultures. Proc. Soc. exp. Biol. (N.Y.)120, 651 (1965).
Tokumaru, T.: Pseudorabies virus in tissue culture: differentiation of two distinct strains of virus by cytopathogenic pattern induced. Proc. Soc. exp. Biol. (N.Y.)96, 55 (1957).
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Poste, G. Characterization of a new canine herpes virus. Archiv f Virusforschung 36, 147–157 (1972). https://doi.org/10.1007/BF01250305
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DOI: https://doi.org/10.1007/BF01250305