Summary
A simple and unique negative viscosity —positive density gradient has been devised which enables an effective single step purification of foot-and-mouth disease virus (FMDV) directly from cytoplasmic lysates.
Using this gradient system, the recovery of infectious virus was greater than 95%. In addition, the isolated viral preparation was virtually free of contaminating cellular macromolecules. Further, the ratio of the volume of sample to gradient could be varied up to about two with a high degree of recovery and concentration.
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Barzilai, R., Lazarus, L.H. & Goldblum, N. Viscosity-density gradient for purification of foot-and-mouth disease virus. Archiv f Virusforschung 36, 141–146 (1972). https://doi.org/10.1007/BF01250304
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DOI: https://doi.org/10.1007/BF01250304