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Cytotoxische Reaktionen an Sendai-Virus infizierten Zellen

Cytotoxic reactions on cells infected with sendai virus

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Zusammenfassung

Monolayerkulturen von. primären Mäusefibroblasten werden mit Myxovirus parainfluenza 1 (Sendai) infiziert. Während der Virusvermehrung wird homologes Sendai-Immunserum und Komplement zugefügt. An den infizierten Zellen können dann parallel zum Auftreten hämadsorbierender Oberflächenantigene immuncytotoxische Reaktionen beobachtet werden. Cytotoxicitätsparameter sind die Freisetzung von Laktatdehydrogenase und von3H nach Markierung der Zellen mit3H-Uridin. Die immuncytotoxische Aktivität, die in den IgM-und IgG-Fraktionen lokalisiert ist, läßt sich durch Sendai-Virus absorbieren.

Summary

Monolayer cultures of primary mouse fibroblasts were infected with parainfluenza-1 (Sendai) virus. During virus replication homologous Sendai-immuneserum and complement were added. On infected cells immunocytotoxic reactions could be observed as soon as hemadsorbing surface antigens were appearing. Parameters of cytotoxicity were the liberation of lactic dehydrogenase and the3H-release after labeling the cells with3H-uridine. The immunocytotoxic activity localized in the IgM and IgG fractions could be absorbed by Sendai virus.

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Literatur

  1. Old, J. L., E. Stockert, E. A. Boyse, andG. Geering: A study of passive immunization against a transplanted G + leukemia with specific antiserum. Proc. Soc. exp. Biol. (N.Y.)124, 63 (1967).

    Google Scholar 

  2. Wiktor, T. J., E. Kuwert, andH. Koprowski: Immune lysis of rabies-infected cells. J. Immunol.101, 1271 (1968).

    Google Scholar 

  3. Mannweiler, Kl.: Elektronen-und lichtmikroskopische Untersuchungen bei der Immuncytolyse masernvirusinfizierter Kulturzellen und deren Bedeutung für die Pathogenese viralbedingter Entmarkungskrankheiten. Wien. Z. Nervenheilk./Suppl.I, 83 (1969).

    Google Scholar 

  4. Hirst, G. K., E. R. Rickard, andL. Whitman: A new method for concentrating influenza virus from allantoic fluid. Proc. Soc. exp. Biol. (N.Y.)50, 129 (1942).

    Google Scholar 

  5. Coons, A. H., E. H. Lduc, andJ. M. Connolly: A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. exp. Med.102, 49 (1955).

    Google Scholar 

  6. Wroblewski, F., andJ. S. Ladue: Lactic dehydrogenase activity in blood. Proc. Soc. exp. Biol. (N.Y.)90, 210 (1955).

    Google Scholar 

  7. Meade, R. C., undR. A. Stiglitz: Improved solvent systems for liquid scintillation counting of body fluids and tissues. Int. J. appl. Eadiat.13, 11 (1962).

    Google Scholar 

  8. Drzeniek, R., andR. Rott: The possible role of lipid containing RNA viruses for the etiology of autoimmune diseases. Bayer-SymposiumI, 245 (1969).

    Google Scholar 

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Author notes

  1. F. Kieling, G. Bandlow & R. Thomssen

    Present address: Hygiene-Institut der Georg-August-Universität Göttingen, BRD

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    1. F. Kieling
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    2. G. Bandlow
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    3. R. Thomssen
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    Additional information

    Gefördert mit Hilfe von Forschungsmitteln des Landes Niedersachsen.

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    Kieling, F., Bandlow, G. & Thomssen, R. Cytotoxische Reaktionen an Sendai-Virus infizierten Zellen. Archiv f Virusforschung 36, 123–132 (1972). https://doi.org/10.1007/BF01250302

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    • DOI: https://doi.org/10.1007/BF01250302

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