Summary
The use of a direct fluorescent antibody technique for the demonstration of Egtved virus in tissue sections from diseased trout and in infected cell cultures is described.
Specific fluorescence indicating the presence of Egtved virus could be demonstrated in kidney, spleen, liver, and heart from fish attacked by VHS.
In cell cultures Egtved virus could be demonstrated eight hours after infection as a granular, mainly cytoplasmic fluorescence resembling the one described for vesicular stomatitis virus.
Another trout pathogen, IPN virus, could be demonstrated in cell cultures by means of a similar technique. No cross-reaction between VHS and IPN conjugates was observed.
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References
David-West, T. S., andN. A. Labzoffsky: Studies on the site of replication of vesicular stomatitis virus. Arch. ges. Virusforsch.24, 30–47 (1968).
Gravell, M., andR. G. Malsberger: A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci.126, 555–565 (1965).
Jensen, M. H.: Preparation of fish tissue cultures for virus research. Bull. Off. int. Epiz.59, 131–134 (1963).
Jensen, M. H.: Research on the virus of Egtved disease. Ann. N.Y. Acad. Sci.126, 422–426 (1965).
Jórgensen, P. E. Vestergård: Serological identification of Egtved virus. Bull. Off. int. Epiz.69, 985–989 (1969).
Jørgensen, P. E. Vestergård, andF. Bregnballe: Infectious pancreatic necrosis in rainbow trout in Denmark. Nord. Vet.-Med.21, 142–148 (1969).
Meyling, A., andV. Bitsch: The diagnosis of pseudorabies by the fluorescent antibody technique. Acta vet. scand.8, 360–368 (1967).
Nairn, R. C.: Fluorescent Protein Tracing. Edinburgh and London: S. Livingstone Ltd., Ed. S. Livingstone Ltd., 1969.
Rodriguez, J., andF. Deinhardt: Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology12, 316–317 (1960).
Rygaard, J., andW. Olsen: Interference filters for improved immunofluorescence microscopy. Acta path. microbiol. scand.,76, 146–148 (1969).
Schäperclaus, W.: Fischkrankheiten, 3. Aufl., Berlin: Akademie-Verlag, 1954.
Voss, H., G. Henneberg, G. Hermann, H. Pichl, S. Schulte-Overberg undH. Werner: Die, Kopplung von Immunseren mit Fluoresceinisothiocyanat. Zbl. Bakt. I. Abt. Orig.203, 249–276 (1967).
Wolf, K., andM. C. Quimby: Established eurythermic line of fish cellsin vitro. Science135, 1065–1066 (1962).
Wolf, K., andP. E. Vestergård Jørgensen: Salmonid viruses: Double infection of RTG-2 cells with Egtved and infectious pancreatic necrosis viruses. Arch. ges. Virasforsch.29, 337–342 (1970).
Zwillenberg, L. O., M. H. Jensen, andHelga H. L. Zwillenberg: Electron microscopy of the virus of viral hemorrhagical septicaemia of rainbow trout (Egtved virus). Arch. ges. Virusforsch.17, 1–19 (1965).
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Jørgensen, P.E.V., Meyling, A. Egtved virus: Demonstration of virus antigen by the fluorescent antibody technique in tissues of rainbow trout affected by viral haemorrhagic septicaemia and in cell cultures infected with Egtved virus. Archiv f Virusforschung 36, 115–122 (1972). https://doi.org/10.1007/BF01250301
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DOI: https://doi.org/10.1007/BF01250301