Summary
Experiments using poly (I-3H) · poly (C) in the presence of various polycationic compounds showed a direct relationship between the degree of adsorption of poly (I) · poly (C) to L cells and the interferon titer determined in the cell culture medium. Deviations from this phenomenon can be explained by differences in toxicity of the polycations and the inhibition of cellular protein synthesis related to this effect.
The degradation of poly (I-3H) · poly (C) by the RNases of the cell culture system is influenced by the presence of the polycations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Billiau, A., C. E. Buckler, F. Dianzani, C. Uhlendorf, andS. Baron: Induction of the Interferon mechanism by single-stranded RNA: Potentiation by polybasic substances. Proc. Soc. exp. Biol. (N.Y.)132, 790–796 (1969).
Colby, C., andM. J. Chamberlin: The specificity of interferon induction in chick embryo cells by helical RNA. Proc. nat. Acad. Sci. (Wash.)63, 160–167 (1969).
De Clercq, E., andT. C. Merigan: Requirement of a stable secondary structure for the antiviral activity of polynucleotides. Nature (Lond.)222, 1148–1152 (1969).
Dianzani, F., P. Cantagalli, S. Gagnoni, andG. Rita: Effect of DEAE-dextran on production of interferon induced by synthetic double-stranded RNA in L cell cultures. Proc. Soc. exp. Biol. (N.Y.)128, 708–710 (1968).
Dianzani, F., G. Rita, P. Cantagalli, andS. Gagnoni: Effect of DEAE-dextran on interferon production and protective effect in mice treated with the double-stranded polynucleotide complex polyinosmic-polycytidylic acid. J. Immunol.102, 24–27 (1969).
Haurowitz, F.: The Chemistry and Function of Proteins, pp. 190 and 205, New York and London: Academic Press 1963.
Koch, G., andJ. M. Bishop: The effect of polycations on the interaction of viral RNA with mammalian cells: Studies on the infectivity of single-and double-stranded poliovirus RNA. Virology35, 9–17 (1968).
Lampson, P., A. A. Tytell, A. K. Field, M. M. Nemes, andM. R. Hilleman: Influence of polyamines on induction of interferon and resistance to viruses by synthetic polynucleotides. Proc. Soc. exp. Biol. (N.Y.)132, 212–218 (1969).
Lauschke, U., E.Lodemann und A.Wacker: Zur Tritiummarkierung von Purinpolyribonucleotiden. (To be published.)
Lodemann, E. undA. Wacker: Interferoninduktion durch Poly(A) · 2Poly(I). Naturwissenschaften57, 673 (1970).
Lodemann, E., and J.Diederich, unpublished results.
Lodemann, E., J.Diederich, H.Feller, and A.Wacker: Induction of interferon in L cells by polyinosinic-polycytidylic acid in the presence of cationic compounds. II. Importance of DEAE-dextran concentration relative to poly(I) · poly(C) concentration. In preparation.
Maes, R. F., andM. V. Fernandes: Interaction between DEAE-dextran and nucleic acids. II. Effect on interferon productionin vivo. Arch. ges. Virusforsch.30, 147–154 (1970).
Mandell, J. D., andA. D. Hershey: A fractionating column for analysis of nucleic acids. Analyt. Biochem.1, 66–77 (1960).
Merigan, T. C., E. De Clercq andG. H. Bausek: Nonviral interferon inducers. J. gen. Physiol.56, 57–70 (1970).
Nomura, S.: Interaction of respiratory syncytial virus with polyions: Enhancement of infectivity with diethylaminoethyl dextran. Proc. Soc. exp. Biol. (N.Y.)128, 163–166 (1968).
Rice, J. M., W. Turner, M. A. Chirigos, andN. R. Rice: Enhancement by poly-D-lysine of poly IC-induced interferon production in mice. Appl. Microbiol.19, 867–869 (1970).
Totoshima, K., andP. K. Vogt: Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology38, 414–426 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wacker, A., Lodemann, E., Diederich, J. et al. Induction of interferon in L cells by polyinosinic-polycytidylic acid in the presence of cationic compounds. Archiv f Virusforschung 36, 71–79 (1972). https://doi.org/10.1007/BF01250297
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01250297