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Production of high titer eastern equine encephalomyelitis virus and viral antigens in chick embryo suspension cultures

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Summary

The growth of eastern equine encephalomyelitis virus in concentrated chick embryo suspension cultures was studied. It was shown that chick embryo cells in serum-free medium, infected at the time of planting, produced up to 1011.5 plaque forming units per ml of virus. This material contained hemagglutinating and complement fixing antigens with titers of 1/10240 and 1/16, respectively. Infectious virus was inactivated at 41 °C and then lyophilized without altering the antigen titers. Hemagglutinating and complement fixing antigens were stable in the liquid state for at least 10 months at 4–6°C.

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Author notes

  1. A. White

    Present address: Department of Biologies Research, Walter Reed Army Institute of Research, Washington, D. C., USA

Authors and Affiliations

  1. Department of Biologies Research, Walter Reed Army Institute of Research, Washington, D. C., USA

    S. Rourke, S. Berman & J. P. Lowenthal

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  1. A. White
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  2. S. Rourke
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  3. S. Berman
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  4. J. P. Lowenthal
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White, A., Rourke, S., Berman, S. et al. Production of high titer eastern equine encephalomyelitis virus and viral antigens in chick embryo suspension cultures. Archiv f Virusforschung 36, 13–17 (1972). https://doi.org/10.1007/BF01250290

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  • DOI: https://doi.org/10.1007/BF01250290

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