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Zur Kontamination von Gewebekulturen mit Mykoplasmen

On contamination of tissue cultures with mycoplasmas

II. Nachweis und serologische Identifizierung der Mykoplasmen

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Zusammenfassung

Von 50 permanenten Gewebekulturen aus 15 verschiedenen Laboratorien waren 35 Kulturen aus 14 Laboratorien mit Mykoplasmen kontaminiert. Von 29 serologisch differenzierten Mykoplasmastämmen gehörten 22 zu Mycoplasma hominis Typ 1 und 7 zu Mycoplasma orale Typ 1. In 8 Laboratorien wurde in den dort befindlichen Zellstämmen ein einheitlicher Kontaminant nachgewiesen. Neben einer Verbreitung der Mykoplasmen innerhalb eines Laboratoriums von Zellkultur zu Zellkultur kommt auch ihrer Verschleppung durch Versand kontaminierter Zellkulturen in andere Institute große Bedeutung zu. Die vorliegenden Untersuchungen unterstreichen die Notwendigkeit, regelmäßig Sterilitätskontrollen auf Mykoplasmen durchzuführen. Bei der noch hohen Rate an Mykoplasmakontaminationen in permanenten Gewebekulturen sind energische Bekämpfungsmaßnahmen erforderlich.

Summary

Out of 50 permanent tissue cultures from 15 different laboratories 35 cultures from 14 laboratories were contaminated with mycoplasmas. Out of 29 serologically differentiated mycoplasma strains 22 belonged tomycoplasma hominis type 1, and 7 tomycoplasma orale type 1. In eight laboratories all cell lines used were contaminated by only one mycoplasma species.

In addition to a spreading of the mycoplasmas from cell culture to cell culture within one laboratory, the possible displacement of contaminated tissue cultures to other institutes by shipment also should be considered. In respect to the still high rate of mycoplasma contaminations in permanent tissue cultures, the present examinations, therefore, underline the necessity to check cell cultures periodically for the presence of mycoplasmas and the requirements of vigorous and effective control means.

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Author notes

  1. W. Witzleb

    Present address: Institut für Medizinische Mikrobiologie und Epidemiologie der Medizinischen Akademie Erfurt, DDR

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  1. Institut für Medizinische Mikrobiologie und Epidemiologie der Medizinischen Akademie Erfurt, DDR

    Th. Blumöhr, H. Dziambor & H. Schweizer

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Für ihre wertvolle technische Assistenz gilt Frau Ch. Adam unser besonderer Dank.

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Witzleb, W., Blumöhr, T., Dziambor, H. et al. Zur Kontamination von Gewebekulturen mit Mykoplasmen. Archiv f Virusforschung 30, 121–129 (1970). https://doi.org/10.1007/BF01250179

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