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Zur Kontamination von Gewebekulturen mit Mykoplasmen

On contamination of tissue cultures with mycoplasmas

I. Beeinträchtigung der Zellphysiologie und die Auswirkungen auf die virologische Diagnostik (Literaturübersicht)

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Zusammenfassung

Mykoplasmakontaminationen von Gewehekulturen führen zu Störungen des Zellwachstums, zu zytopathischen Effekten unterschiedlichen Grades, zu groben Veränderungen der biochemisch erfaßbaren Lebensäußerungen der Zelle und zu eindeutigen Schädigungen des Nukleinsäurestoffwechsels. Die Nachteile für die virologische Diagnostik liegen in einer unterschiedlich starken Veränderung der Virusvermehrung in der Zelle, vor allem aber sind Störungen von serologisch-virologischen Untersuchungen durch das Mykoplasma-Antigen in der Gewebekultur zu erwarten. Bei Anzüchtungsversuchen besteht auf Grund der mehr oder weniger stark ausgeprägten zytopathogenen Aktivität und anderen Eigenschaften der Mykoplasmen die Gefahr der Verwechslung mit Viren.

Summary

In tissue cultures, presence of mycoplasmas may cause alterations of cell growth, cytopathic effects of varying degrees, and heavy changes in cellular physiology, especially in respect to nucleic acid metabolism. Thus, the use of contaminated cultures for virological diagnostic procedures results in a broad and inpredictable variation of virus multiplication but due to the presence of the mycoplasma antigen, main disadvantages are to be expected during serological-virological examinations. Furthermore, owing to the more or less distinct cytopathogenic activity and other properties of the mycoplasmas, there is the danger of taking them as viruses in culture tests.

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Literatur

  1. Afshar, A.: The growth of Mycoplasma bovigenitalium in cell cultures. J. gen. Microbiol.47, 103–110 (1967).

    Google Scholar 

  2. Bailey, J. S., H. W. Clark, W. R. Felts, R. C. Fowler, andT. McP. Brown: Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J. Bact.82, 542–547 (1961).

    Google Scholar 

  3. Barile, M. F., W. F. Malizia, andD. B. Riggs: Incidence and detection of pleuropneumonia-like organisms in cell cultures by fluorescent antibody and cultural procedures. J. Bact.84, 130–136 (1962).

    Google Scholar 

  4. Brownstein, B., andA. F. Graham: Interaction of Mengo virus with L cells. Virology14, 303–311 (1961).

    Google Scholar 

  5. Buskirk, H. H.: Control of pleuropneumonia-like organisms in cell cultures. Appl. Microbiol.15, 1442–1446 (1967).

    Google Scholar 

  6. Butler, M.: Diskussion. Internationales Symposium über Mykoplasmaerkrankungen des Menschen. Reinhardsbrunn 1968.

  7. Butler, M., andR. H. Leach: A mycoplasma which induces acidity and cytopathic effect in tissue culture. J. gen. Microbiol.34, 285–294 (1964).

    Google Scholar 

  8. Butler, M., andR. H. Leach: Mycoplasma associated with tissue cultures, leukaemia and human tumours. Proc. roy. Soc. Med.59, 1116–1117 (1966).

    Google Scholar 

  9. Carski, T. R., andC. C. Shepard: Pleuropneumonia-like (mycoplasma) infections of tissue culture. J. Bact.81, 626–635 (1961).

    Google Scholar 

  10. Chang, R. S.: Differences in inositol requirements of several strains of HeLa, conjunctival and amnion cells. Proc. Soc. exp. Biol. (N.Y.)99, 99–102 (1958).

    Google Scholar 

  11. Chao, F. C., F. Freeman, J. G. Cummings, andB. J. Berridge, Jr.: Amino acids of the ornithine cycle in transformed hamster fibroblasts carrying pleuropneumonia-like organisms. Cancer Res.27, 1474–1481 (1967).

    Google Scholar 

  12. Collier, L. H.: Contamination of stock lines of human carcinoma cells by pleuropneumonia-like organisms. Nature (Lond.)180, 757–758 (1957).

    Google Scholar 

  13. Copperman, R., andH. E. Morton: Reversible inhibition of mitosis in lymphocyte cultures by non-viable mycoplasma. Proc. Soc. exp. Biol. (N.Y.)123, 790–795 (1966).

    Google Scholar 

  14. Coriell, L. L., D. P. Fabrizio, andS. R. Wilson: Comparison of pleuropneumonia-like organism strains from tissue culture by complement fixation. Ann. N.Y. Acad. Sci.79, 574–580 (1960).

    Google Scholar 

  15. Coriell, L. L., M. G. Tall, andH. Gaskill: Common antigens in tissue culture cell lines. Science128, 198–199 (1958).

    Google Scholar 

  16. Cross, G. F., M. R. Goodman, andE. J. Shaw: Detection and treatment of contaminating mycoplasmas in cell culture. Aust. J. exp. Biol. med. Sci.45, 201–212 (1967).

    Google Scholar 

  17. Eaton, M. D., G. Meiklejohn, andW. van Herick: Studies on the etiology of primary atypical pneumonia, a filterable agent transmissible to cotton rats, hamsters, and chick embryos. J. exp. Med.79, 649–668 (1944).

    Google Scholar 

  18. Fogh, J., andH. Fogh: Chromosome changes in PPLO-infected FL human amnion cells. Proc. Soc. exp. Biol. (N.Y.)119, 233–238 (1965).

    Google Scholar 

  19. Fogh, J., andC. Hacker: Elimination of pleuropneumonia-like organisms from cell cultures. Exp. Cell Res.21, 242–244 (1960).

    Google Scholar 

  20. Fogh, J., E. Hahn, andH. Fogh: Effects of pleuropneumonia-like organisms on cultured human cells. Exp. Cell Res.39, 554–566 (1965).

    Google Scholar 

  21. Girardi, A. J., V. V. Hamparian, N. L. Somerson, andL. Hayflick: Mycoplasma isolates from primary cell cultures and human diploid cell strains. Proc. Soc. exp. Biol. (N.Y.)120, 760–771 (1965).

    Google Scholar 

  22. Girardi, A. J., L. Hayflick, A. M. Lewis, andN. L. Somerson: Recovery of mycoplasmas in the study of human leukemia and other malignancies. Nature (Lond.)205, 188–189 (1965).

    Google Scholar 

  23. Grumbles, L. C., C. F. Hall, andG. Cummings: Characteristics of avian mycoplasma (PPLO) in tissue cultures of human and avian cells. Avian Dis.8, 274–280 (1964).

    Google Scholar 

  24. Hakala, M. T., J. F. Holand, andJ. S. Horoszewicz: Change in pyrimidine deoxyribonucleoside metabolism in cell culture caused by mycoplasma (PPLO) contamination. Biochem. biophys. Res. Commun.11, 466–471 (1963).

    Google Scholar 

  25. Hayflick, L.: Decontaminating tissue cultures infected with pleuropneumonia-like organisms. Nature (Lond.)185, 783–784 (1960).

    Google Scholar 

  26. Hayflick, L.: Tissue cultures and mycoplasmas. Tex. Rep. Biol. Med., Suppl. 1 to23, 285–303 (1965).

    Google Scholar 

  27. Hayflick, L., andW. R. Stinebring: Intracellular growth of pleuropneumonia-like organisms. Anat. Rec.121, 447 (1955).

    Google Scholar 

  28. Hayflick, L., andW. R. Stinebring: Intracellular growth of pleuropneumonia-like organisms (PPLO) in tissue culture and in ovo. Ann. N.Y. Acad. Sci.79, 433–449 (1960).

    Google Scholar 

  29. Hayflick, L., W. R.Stinebring, F. C.Breckenridge, and C. M.Pomerat: Some effects of human pleuropneumonia-like organisms on tissue cultures of human synovial cells. Bact. Proc.83 (1956).

  30. Hearn, H. J., J. E. Officer, V. Elsner, andA. Brown: Detection, elimination and prevention of contamination of cell cultures with pleuropneumonia-like organisms. J. Bact.78, 575–582 (1959).

    Google Scholar 

  31. Herderscheê, D., A. C. Ruys, andG. R. van Rhijn: Pleuropneumonia-like organisms in tissue culture. Antony v. Leeuwenhoek29, 368–376 (1963).

    Google Scholar 

  32. Hlinak, P., undD. Schimmel: Kontaminationen von Zellkulturen durch Mykoplasmen. 3. Mitteilung: Beeinflussung von Mykoplasmen in kontaminierten Zellkulturen. Mh. Vet.-Med.23, 259–264 (1968).

    Google Scholar 

  33. Hummeler, K., D. Armstrong, andN. Tomassini: Cytopathogenic mycoplasmas associated with two human tumors. II. Morphological aspects. J. Bact.90, 511–516 (1965).

    Google Scholar 

  34. Jézéquel, A.-M., M. M. Shreeve, andJ. W. Steiner: Segregation of nucleolar components in mycoplasma-infected cells. Lab. Invest.16, 287–304 (1967).

    Google Scholar 

  35. Kagan, G. Ja., und I. V.Rakowskaja: Mykoplasmainfektionen in Gewebekulturen (russ.). Leningrad (1968).

  36. Kenny, G. E.: The effect of pleuropneumonia-like organisms (mycoplasma) on growth of mammalian cells in vitro. Ph. D. Thesis, University of Minnesota, Minneapolis 1961.

    Google Scholar 

  37. Kenny, G. E., andM. E. Pollock: Mammalian cell cultures contaminated with pleuropneumonia-like organisms. I. Effect of pleuropneumonia-like organisms on growth of established cell strains. J. infect. Dis.112, 7–16 (1963).

    Google Scholar 

  38. Kraemer, P. M.: Mycoplasma (PPLO) from covertly contaminated tissue cultures: Differences in arginine degradation between strains. Proc. Soc. exp. Biol. (N.Y.)117, 910–918 (1964).

    Google Scholar 

  39. Leach, R. H., andM. Butler: Comparison of mycoplasmas associated with human tumors, leukemia, and tissue cultures. J. Bact.91, 934–941 (1966).

    Google Scholar 

  40. Leach R. H., and F. D.Hargreaves: The influence of different mycoplasma species on the growth of several viruses in tissue culture. Internationales Symposium über Mykoplasmaerkrankungen des Menschen. Reinhardsbrunn 1968.

  41. MacPherson, I.: Mycoplasmas in tissue culture. J. Cell Sci.1, 145–168 (1966).

    Google Scholar 

  42. Nardone, R. M., J. Todd, P. Gonzalez, andE. V. Gaffney: Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms. Science149, 1100–1101 (1965).

    Google Scholar 

  43. Negroni, G.: Isolation of viruses from leukaemic patients. Brit. med. J.1, 927–929 (1964).

    Google Scholar 

  44. O'Connell, R. C., R. G. Wittler, andJ. E. Faber: Aerosols as a source of widespread mycoplasma contamination of tissue cultures. Appl. Microbiol.12, 337–342 (1964).

    Google Scholar 

  45. O'Malley, J. P., Z. A. McGee, M. F. Barile, andL. F. Barker: Identification of the A-1 agent as Mycoplasma gallisepticum. Proc. nat. Acad. Sci. (Wash.)56, 895–901 (1966).

    Google Scholar 

  46. Paton, G. R., J. P. Jacobs, andF. T. Perkins: Chromosome changes in human diploid-cell cultures infected with mycoplasma. Nature (Lond.)207, 43–45 (1965).

    Google Scholar 

  47. Perlman, D., S. B. Rahman, andJ. B. Semar: Antibiotic control of mycoplasma in tissue culture. Appl. Microbiol.15, 82–85 (1967).

    Google Scholar 

  48. Pollock, M. E., andG. E. Kenny: Mammalian cell cultures contaminated with. PPLO. III. Elimination of PPLO with specific antiserum. Proc. Soc. exp. Biol. (N.T.)112, 176–181 (1963).

    Google Scholar 

  49. Pollock, M. E., G. E. Kenny, andJ. T. Syverton: Isolation and elimination of pleuropneumonia-like organisms from mammalian cell cultures. Proc. Soc. exp. Biol. (N.Y.)105, 10–15 (1960).

    Google Scholar 

  50. Pollock, M. E., P. E. Treadwell, andG. E. Kenny: Mammalian cell cultures contaminated with pleuropneumonia-like organisms. II. Effect of PPLO on cell morphology in established monolayer cultures. Exp. Cell Res.31, 321–328 (1963).

    Google Scholar 

  51. Pontén. J., andI. MacPherson: Interference with Rous sarcoma virus focus formation by a mycoplasma-like factor present in human cell cultures. Ann. Med. exp. Fenn.44, 260–264 (1966).

    Google Scholar 

  52. Purcell, R. H., N. L. Somerson, H. Fox, D. C. Wong, H. C. Turner, andR. M. Chanock: Identification of acid-inducing agent and related mycoplasma as Mycoplasma hyorhinis. J. nat. Cancer Inst.37, 251–253 (1966).

    Google Scholar 

  53. Robinson, L. B., R. H. Wichelhausen, andB. Roizman: Contamination of human cell cultures by pleuropneumonia organisms. Science124, 1147 (1956).

    Google Scholar 

  54. Rothblat, G. H.: PPLO contamination in tissue cultures. Ann. N.Y. Acad. Sci.79, 430–432 (1960).

    Google Scholar 

  55. Rothblat, G. H., andH. E. Morton: Detection and possible source of contamination of pleuropneumonia-like organisms (PPLO) in cultures of tissue cells. Proc. Soc. exp. Biol. (N.Y.)100, 87–90 (1959).

    Google Scholar 

  56. Rouse, H. C, and V. H.Bonifas: Depletion by pleuropneumonia-like organisms of arginine required for adenovirus plaque formation. Bact. Proc. 147 (1962).

  57. Rouse, H. C., V. H. Bonifas, andR. W. Schlesinger: Dependence of adenovirus replication on arginine and inhibiton of plaque formation by pleuropneumonia-like organisms. Virology20, 357–365 (1963).

    Google Scholar 

  58. Russell, W. C.: Alterations in the nucleic acid metabolism of tissue culture cells infected by mycoplasmas. Nature (Lond.)212, 1537–1540 (1966).

    Google Scholar 

  59. Sabin, A. B.: Nature and source of mycoplasma in various tissue cultures. Ann. N.Y. Acad. Sci.143, 628–634 (1967).

    Google Scholar 

  60. Schimke, R. T.: Studies on the metabolism of arginine by mycoplasma. Ann. N.Y. Acad. Sci.143, 573–577 (1967).

    Google Scholar 

  61. Schimke, R. T., andM. F. Barile: Arginine metabolism in pleuropneumonia-like organisms isolated from mammalian cell cultures. J. Bact.86, 195–206 (1963).

    Google Scholar 

  62. Schimke, R. T., andM. F. Barile: Arginine breakdown in mammalian cell cultures contaminated with pleuropneumonia-like organisms (PPLO). Exp. Cell Res.30, 593–596 (1963).

    Google Scholar 

  63. Schimmel, D. undP. Hlinak: Kontaminationen von Zellkulturen durch Mykoplasmen. 1. Mitteilung: Über Isolierung und Morphologie von Mykoplasmen aus permanenten Zellkulturen. Mh. Vet.-Med.21, 64–69 (1966).

    Google Scholar 

  64. Schimmel, D., undP. Hlinak: Kontaminationen von Zellkulturen durch Mykoplasmen. 2. Mitteilung: Über die Isolierung von Mykoplasmen aus Primärkulturen und verschiedenem Organmaterial. Mh. Vet.-Med.22, 387–390 (1967).

    Google Scholar 

  65. Schweizer, H., W. Witzleb undTh. Blumöhr: Zur Kontamination von Gewebekulturen mit Mykoplasmen. III. Eliminierung von Mykoplasmen aus kontaminierten Gewebekulturen. Arch. ges. Virusforsch.30, 130 (1970).

    Google Scholar 

  66. Scriba, M.: Versuche zur Beseitigung von Mykoplasmakontaminationen in Zellkulturen durch Antibiotika. Z. med. Mikrobiol. Immunol.154, 267–276 (1968).

    Google Scholar 

  67. Somerson, N. L., andM. K. Cook: Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bact.90, 534–540 (1965).

    Google Scholar 

  68. Stanbridge, E.: Diskussion. Internationales Symposium über Mykoplasmaerkrankungen des Menschen. Reinhardsbrunn 1968.

  69. Stanbridge, E., M.Önen, and L.Hayflick: Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasmas. Internationales Symposium über Mykoplasmaerkrankungen des Menschen. Reinhardsbrunn 1968.

  70. Witzleb, W., Th. Blumöhr, H. Dziambor undH. Schweizee: Zur Kontamination von Gewebekulturen mit Mykoplasmen. II. Nachweis und serologische Identifizierung der Mykoplasmen. Arch. ges. Virusforsch.30, 121–129 (1970).

    Google Scholar 

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  1. W. Witzleb

    Present address: Institut für Medizinische Mikrobiologie und Epidemiologie der Medizinischen Akademie Erfurt, DDR

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    Witzleb, W. Zur Kontamination von Gewebekulturen mit Mykoplasmen. Archiv f Virusforschung 30, 113–120 (1970). https://doi.org/10.1007/BF01250178

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