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Avian sarcoma virus transformed hamster cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity

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Summary

Hamster cells transformed with the Schmidt-Ruppin strain of avian sarcoma virus were selected for resistance to 5-bromodeoxyuridine (BUDR). The resistant cell lines Ha(SR)BU-25 and Ha(SR)BU-100, proliferated in the presence of 25 and 100 μg/ml BUDR, respectively. The resistant cells were deficient in thymidine kinase activity. They did not grow in HATG medium and did not incorporate labelled thymidine into DNA.

Several single-cell clones were isolated in medium containing 100 μg/ml BUDR from Ha(SR)BU-100 cells. These isolated cell clones differed in morphology and modal number of chromosomes from each other. None of the clones incorporated thymidine into DNA.

The BUDR resistant cells were tested for their tumorigenicity in young hamsters. The number of cells needed for induction of tumor growth was higher with the BUDR resistant cells than with original clone of Ha(SR) cells.

All clones of thymidine kinase deficient cells were tested for the presence of the virus genome by fusion with chicken embryo cells. All clones of Ha(SR)BU-100 cells contained the virus genome, and infectious virus could be rescued from these cells.

Therefore, the genome of avian sarcoma virus can persist in virogenic hamster cells growing in the presence of 5-bromodeoxyuridine. These virogenic cells deficient in thymidine kinase activity are useful for preparation of cell hybrids.

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Author notes

  1. C. Altaner & H. Schlechte

    Present address: Cancer Research Institute, Slovak Academy of Science, Bratislava, Czechoslovakia

Authors and Affiliations

  1. Cancer Research Institute, Slovak Academy of Science, Bratislava, Czechoslovakia

    M. Hladka

  2. Forschungszentrum für Molekular-Biologie und Medizin, Abt. Somatische Zellgenetik der DAdW, Berlin-Buch, DDR

    H. Schlechte

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  1. C. Altaner
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  2. M. Hladka
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  3. H. Schlechte
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Altaner, C., Hladka, M. & Schlechte, H. Avian sarcoma virus transformed hamster cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity. Archiv f Virusforschung 41, 40–51 (1973). https://doi.org/10.1007/BF01249927

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  • DOI: https://doi.org/10.1007/BF01249927

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