Summary
We have demonstrated that the small plaque (SP) variant of Sindbis virus is more extensively neutralized by complete antibody and serum IgG fractions than is the large plaque (LP) variant. The Fc portion failed to demonstrate any neutralizing potential and did not block subsequent neutralization by IgG or Fab fragments. The LP and SP variants of Sindbis gave identical patterns upon isoelectric focusing. Treatment of the virus with intact serum IgG or isolated Fab fragments was found not to have an irreversible effect on the net charge of the virus population. The selective processes involved in the maturation of the nucleocapsid implicate the incorporation of charged host determinants which are then reflected in the charge of the virion. It is suggested that the virus core has a preferential affinity for those components of the plasma membranes which have an isoelectric point at pH 3.6.
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Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Ann. Biochem.1, 279–285 (1960).
Breeze, D. C., andH. V. Thorne: The electrophoretic separation of two plaquetype variants of EMC virus. Nature (Lond.)210, 1080–1084 (1966).
Bres, P.: Quelques propriétés des variants du virus Sindbis, à grandes et à petites plages, inoculés au souriceau. Ann. Inst. Pasteur109, 866–873 (1965).
Bose, H. R., andB. P. Sagik: Immunological activity associated with the nucleocapsid and envelope components of an arbovirus. J. Virol.5, 410–412 (1970).
Bose, H. R., G. Z. Carl, andB. P. Sagik: Separation of Sindbis virus plaque type variants by calcium phosphate chromatography. Arch. ges. Virusforsch.29, 83–87 (1970).
Bose, H. R., M. A. Brundige, G. Z. Carl, andB. P. Sagik: Peptide composition of Sindbis virus variants. Arch. ges. Virusforsch.31, 207 (1970).
Burge, B. W., andJ. H. Strauss: Glycopeptides of the membrane glycoprotein of Sindbis virus. J. molec. Biol.47, 449–454 (1970).
Burnet, F. M., E. V. Keogh, andD. Lush: Immunological reactions of filterable viruses. Aust. J. exp. Biol. med. Sci.15, 227 (1937).
Cowan, K. M., andJ. H. Graves: Immunochemical studies of foot-and-mouth disease virus. III. Acridine orange staining of agar gel precipitation reactions. Virology34, 544–548 (1968).
Cramer, N., E. H. Lennette, S. J. Hagens, andF. Y. Fujimoto: Difference in mechanism of viral neutralization underin vitro andin vivo conditions. J. Immunol.96, 284–288 (1966).
Douglas, H. W., B. L. Williams, andC. J. M. Rondle: Microelectrophoresis of pox viruses in molar sucrose. J. gen. Virol.5, 391–396 (1969).
Dulbeoco, R., andM. Vogt: One-step growth curve of western equine encephalomyelitis virus on chicken embryo cells grownin vitro and analysis of virus yields from single cells. J. exp. Med.99, 183–199 (1954).
Eagle, H.: Nutrition needs of mammalian cells in tissue culture. Science.122, 501–504 (1955).
Hanks, J. H., andR. E. Wallace: Relation of oxygen and pressure in the preservation of tissues by refrigeration. Proc. Soc. exp. Biol. (N.Y.)71, 196–200 (1949).
Hannoun, C., J. Cisso, andP. Andoin: Mutants à petites plaques du virus Sindbis. Ann. Inst. Pasteur.107, 598–603 (1964).
Keckwick, R. A.: The serum proteins in multiple myelomatoses. Biochem. J.34, 1248–1256 (1940).
Klinman, R., C. A. Long, andF. Karush: The role of antibody bivalence in the neutralization of bacteriophage. J. Immunol.99, 1128–1137 (1967).
Mandy, W. J., M. K. Stambaugh, andA. Nissonoff: Amino acid composition of univalent fragments of rabbit antibody. Science.140, 901–903 (1963).
McConahey, P. J., andF. J. Dixon: A method of trace iodination of proteins for immunologic studies. Int. Arch. Allergy.29, 185–189 (1966).
Müller-Eberhard, H. J.: A new supporting medium for preparative electrophoresis. Scand. J. clin. Lab. Invest.12, 33–41 (1960).
Murakami, W. T., R. Fine, M. R. Harrington, andZ. Ben Sassan: Properties and amino acid composition of polyoma virus purified by zonal ultracentrifugation. J. molec. Biol.36, 153–166 (1968).
Oroszlan, S., C. L. Fisher, T. B. Stanley, andR. V. Gilden: Proteins of the murine C-type RNA tumor viruses: Isolation of a group specific antigen by isoelectric focusing. J. gen. Virol.8, 1–10 (1970).
Ouchterlony, O.: Antigen-antibody reactions in gels IV: Types of reactions in coordinated systems of diffusion. Acta path. microbiol. scand.32, 231 (1953).
Pfefferkorn, E. R., andH. S. Hunter: The source of the ribonucleic acid and phospholipid of Sindbis virus. Virology20, 446–456 (1963b).
Porter, R. R.: The hydrolysis of rabbit-globulin and antibodies with crystalline papain. Biochem. J.73, 119–129 (1959).
Pringle, C. R.: Electrophoretic properties of foot-and-mouth disease virus strains and selection of intra-strain mutants. J. gen. Virol.4, 541–555 (1969).
Probstmeyer, F., andL. L. Schechmeister: A comparative study of vesicular stomatitis virus and its plaque type mutant. Arch. ges. Virusforsch.23, 79–88 (1968).
Purifoy, D. J. M., J. A. Purifoy, andB. P. Sagik: A mathematical analysis of concomitant virus replication and heat inactivation. J. Virol.2, 275–280 (1968).
Reinarz, A. B. G., M. G.Broome, and B. P.Sagik: Age dependent resistance of mice to Sindbis virus infections. I. Replication of large and small plaque forming variants as a function of host age. Infect. and Immunity. In Press. (1970).
Rosenberg, M. D.: Plasma membranes of the liver cells of chick embryos. I. Isolation procedures. Biochim. biophys. Acta (Amst.)173, 11–22 (1969).
Scheidegger, J. J.: Une micro-methode de l'immuno-électrophorèse. Int. Arch. Allergy7, 103–107 (1955).
Strauss, A. J. L., B. C. Seegal, K. C. Hsu, P. M. Burkholder, W. L. Nastuk, andK. F. Osserman: Immunofluorescence demonstration of a muscle-binding, complement-fixing serum globulin fraction in myasthenia gravis. Proc. Soc. exp. Biol. (N.Y.)105, 184–191 (1960).
Strauss, J. H., B. W. Burge, andJ. E. Darnell: Sindbis virus infection of chick and hamster cells: Synthesis of virus specific proteins. Virology37, 367–376 (1969).
Swensson, H.: Isoelectric fractionation, analysis and characterization of ampholytes in natural pH gradients. III. Description of apparatus for electrolysis in columns stabilized by density gradients and direct determination of isoelectric points. Arch. Biochem. Biophys. Suppl.1, 132–150 (1962).
Taylor, R. M., H. S. Hurlburt, T. H. Work, J. R. Kingston, andT. E. Frothingham: Sindbis virus; A newly recognized arthropod-transmitted virus. Amer. J. trop. Med. Hyg.4, 844–862 (1955).
Thorne, H. V., W. Hourse, andA. L. Kisch: Electrophoretic properties and purification of large and small plaque forming strains of polyoma virus. Virology27, 37–44 (1965).
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Pedersen, C.E., Barrera, C.R. & Sagik, B.P. Immunochemical studies of Sindbis virus variants. Archiv f Virusforschung 41, 28–39 (1973). https://doi.org/10.1007/BF01249926
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DOI: https://doi.org/10.1007/BF01249926