Skip to main content
SpringerLink
Log in

Konzentrierung und Reinigung von Maul-und-Klauenseuche-(MKS-) Virus durch Polyäthylenglykol (PEG)

Concentration and purification of foot-and-mouth disease (FMD) virus by polyethylenglycol (PEG)

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Zusammenfassung

MKS-Virus der Typen 0, A und C wurde durch Präzipitation mit 8–10% (w/v) Polyäthylenglycol 6000 sowie nachfolgende Polster- und Zonenzentrifugierung im Saccharosegradienten aus infizierten BHK-Zellkulturen konzentriert und gereinigt. Die durchschnittliche Virusausbeute schwankte zwischen 0,7 und 8 mg/1000 ml infektiösem Kulturmedium, entsprechend 20–37% der Gesamtinfektiosität. In der quantitativen Polyacrylamidgel-Elektrophorese wurde in hochgereinigten Virussuspensionen eine schnell wandernde Proteinkomponente nachgewiesen, die mit markierter Virusnukleinsäure assoziiert war.

Summary

Foot-and-mouth disease virus, strains O1-Kaufbeuren, A2-Spain and C-Oberbayern, respectively, was purified from infected BHK-21 cell cultures by precipitation with polyethylenglycol 6000 to 8 and 10 per cent (w/v) concentration followed by centrifugation through a sucrose cushion and rate zonal centrifugation, Mean virus recovery was 0,7 to 8 rag per 1000 ml of infectious tissue culture fluid representing 20 to 37 per cent of total infectivity. Using highly purified virus suspensions a fast migrating protein component was detected by quantitative polyacrylamide gel electrophoresis which was associated with labelled viral nucleic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  • Arlinghaus, R. B., J. Polatnick, andG. F. Vande Woude: Studies on foot-and-mouth disease virus ribonucleic acid synthesis. Virology30, 541–550 (1966).

    Google Scholar 

  • Bachrach, H. L., J. J. Callis, W. E. Hess, andR. E. Patty: A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction. Virology4, 224–236 (1957).

    Google Scholar 

  • Bachrach, H. L., andJ. Polatnick: Decigram quantities of pure foot-and-mouth disease virus from cell cultures. Bio-med. Engng.10, 589–599 (1968).

    Google Scholar 

  • Brown, F., andB. Cartwright: Purification of radioactive foot-and-mouth disease virus. Nature (Lond.)199, 1168–1170 (1963).

    Google Scholar 

  • Cowan, K. M., andJ. H. Graves: A third antigenic component associated with foot-and-mouth disease infection. Virology30, 528–540 (1966).

    Google Scholar 

  • Davis, B. J.: Disc Electrophoresis. Distillation Products Industries, Rochester, N.Y. (1963).

  • Dietzschold, B., O. R.Kaaden, T.Tokui, and H. O.Böhm: Polynucleotide sequence homologies among the RNA of foot-and-mouth disease virus type A, C and O. J. gen. Virol. (in press).

  • Fayet, M. T.: Concentration du virus de la fièvre aphteuse par le polye, thylene glycol. Ann. Inst. Pasteur118, 356–366 (1970).

    Google Scholar 

  • Hantschel, H., H. Hahnefeld undE. Hahnefeld: Eine einfache Methode zur Anreicherung von Virus aus Gewebekulturflüssigkeit durch Adsorption an Kalziumphosphat. Arch. exp. Vet.-Med.20, 751–766 (1966).

    Google Scholar 

  • Lowry, O. M., N. J. Rosebrough, A. L. Farr, andR. J. Randall: Protein measurements with the folin phenol reagent. J. biol. Chem.193, 265–275 (1951).

    Google Scholar 

  • Matthaeus, W.: Studien über Fällung, Ionenaustauscherchromatografie und elektrophoretische Beweglichkeit von Kulturviren in Gegenwart wasserlöslicher, linearer Polykondensations- und Polymerisationsprodukte. Zbl. Bakt. I. Abt. Orig.210, 1–14 (1969).

    Google Scholar 

  • Rowland, D. J., B. Cartwright, andF. Brown: Evidence for an Internal Antigen in Foot-and Mouth Disease Virus. J. gen. Virol.4, 479–487 (1969).

    Google Scholar 

  • Schwöbel, W., undV. Siedentopf: Charakterisierung eines drei Jahre alten Zellstammes aus der Schweineniere und Untersuchungen über sein Verhalten gegenüber dem Virus der Maul- und Klauenseuche. Zbl. Bakt. I. Abt. Orig.181, 3–16 (1961).

    Google Scholar 

  • Stoker, M., andJ. MacPherson: Studies on transformation of hamster cells by polyoma virusin vitro. Virology14, 359–370 (1961).

    Google Scholar 

  • Strohmaier, K.: Virusconcentration by ultrafiltration. In: Methods of Virology vol. 2 (K. Maramorosch andH. Koprowski, eds.), New York: Academic Press, 1967.

    Google Scholar 

  • Wagner, G. G., J. L. Card, andK. M. Cowan: Immunochemical studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease virus concentrated by polyethylene glycol precipitation. Arch. ges. Virusforsch.30, 343–352 (1970).

    Google Scholar 

Download references

Author information

Author notes

  1. T. Tokui

    Present address: National Institute of Animal Health, Kodaira, Tokyo, Japan

Authors and Affiliations

  1. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, BRD

    O. R. Kaaden, B. Dietzschold, H. D. Matheka & T. Tokui

Authors
  1. O. R. Kaaden
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Dietzschold
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. D. Matheka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Tokui
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaaden, O.R., Dietzschold, B., Matheka, H.D. et al. Konzentrierung und Reinigung von Maul-und-Klauenseuche-(MKS-) Virus durch Polyäthylenglykol (PEG). Archiv f Virusforschung 35, 104–113 (1971). https://doi.org/10.1007/BF01249757

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01249757

Search

Navigation