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Parvoviruses as contaminants of permanent human cell lines

II. Physicochemical properties of the isolated viruses

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Summary

For three reference strains (KBSH, TVX, and Lu III) of the parvoviruses isolated from permanent human cell lines the morphological and physicochemical properties were determined and yielded the following results:

  1. 1.

    The cubic virus particles had a mean diameter of 19–21 mμ. Their capsid is probably constructed from 32 capsomeres and fits the symmetrical requirements of a pentakis dodekahedron.

  2. 2.

    In CsCl the complete infective viruses showed a buoyant density of 1.395 g/ml whereas empty capsids and other hemagglutinating breakdown products banded around 1.31 g/ml. A further virus peak associated with both infective and hemagglutinating properties could be detected at a medium density of 1.35 g/ml.

  3. 3.

    The sedimentation coefficient of complete KBSH-virus was determined to be 105±10 S and hemagglutinating fractions of the agent with a buoyant density around 1.31 g/ml sedimented at 72 S.

  4. 4.

    According to the successful incorporation of3H-thymidine, the inhibition of multiplication by IUDR, and the behaviour during acridine orange staining the viruses contain DNA of single stranded configuration.

  5. 5.

    The viruses proved resistant to the action of ether, chloroform, Freon, Nadeoxycholate, and enzymes such as trypsin, pepsin, papain, RNase, and DNase. Heating of purified preparations to 75 °C for 1 hour also reduced infectivity and hemagglutination titers only a houndred-fold.

When compared with the physicochemical characteristics of well accepted and of some candidate members of the parvovirus group these data clearly allow the classification of the isolated agents into this new viral grouping.

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Author notes

  1. G. Siegl

    Present address: Institute of Hygiene and Medical Microbiology, University of Berne, Switzerland

Authors and Affiliations

  1. Institute of Hygiene and Medical Microbiology, University of Berne, Switzerland

    C. Hallauer, A. Novak & G. Kronauer

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  1. G. Siegl
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  2. C. Hallauer
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  3. A. Novak
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  4. G. Kronauer
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Siegl, G., Hallauer, C., Novak, A. et al. Parvoviruses as contaminants of permanent human cell lines. Archiv f Virusforschung 35, 91–103 (1971). https://doi.org/10.1007/BF01249756

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