Summary
Human lymphocytes stimulated with phytohemagglutinin (PHA) acquired the ability to support the replication of poliovirus. The maximum number of cells producing virus in cultures of lymphocytes coincided with the peak of cellular RNA synthesis induced by PHA stimulation. In cultures infected with a high multiplicity of virus, the PHA-induced DNA synthesis of the lymphocytes was partially inhibited. The inhibition occurred when virus was added up to 3 days after initiation of the PHA-stimulated leukocyte cultures, but not after 3 days. Inhibition of DNA synthesis by poliovirus could also be demonstrated in leukocytes cultivated in the absence of PHA. Pre-incubation of the lymphocytes with poliovirus before stimulation with PHA resulted in enhanced inhibition of DNA synthesis. It is postulated that part of the inhibition of the response of lymphocytes to PHA results from an abortive infection of the cells before new cellular RNA synthesis begins.
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This investigation was supported in part by research contract PH 43-68-678 within the Special Virus-Leukemia Program of the National Cancer Institute, and by research grants HE 05435 from the National Heart Institute and AI 05382 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health.
On leave from the University of Nijmegen, The Netherlands.
Public Health Service Research Career Development Awardee, 5-K3-AI 25,943 from the National Institute of Allergy and Infectious Diseases.
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Willems, F.T.C., Rawls, W.E. Poliovirus inhibition of the phytohemagglutinin response in human lymphocytes. Archiv f Virusforschung 27, 352–363 (1969). https://doi.org/10.1007/BF01249657
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DOI: https://doi.org/10.1007/BF01249657