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The effect of 5-iododeoxyuridine and actinomycin D on the multiplication of junin virus

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Summary

Multiplication of Junin virus in Vero cells was not inhibited neither by 5-iododeoxyuridine nor by actinomycin D indicating that the nucleic acid of the virus is RNA.

Normal yields of virus were obtained although 60 per cent of cellular RNA synthesis was inhibited in control cultures treated with actinomycin D. Same results were observed when a concentration of actinomycin D that inhibits 90 per cent of host cell RNA synthesis in control cells was present during the first 6 hours of the growth cycle.

These results suggest that the replication of Junin virus is not DNA dependent.

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References

  1. Parodi, A. S., C. E. Coto, M. Boxaca, S. Lajmanovich, andS. Gonzales: Characteristics of Junin virus. Arch. ges. Virusforsch.19, 393 (1966).

    Google Scholar 

  2. Coto, C. E., L. Zaharzevskij etA. S. Parodi: Localisation subcellulaire du virus Junin dans le cerveau de souris en lactation. Etude du pouvoir infectant. C. R. Soc. Biol. (Paris)161, 720 (1967).

    Google Scholar 

  3. Zaharzevskij, L., C. E.Coto en A. S.Parodi: Localización subcelular del virus Junin en el cerebro del ratón lactante. Estudio de microscopía electrónica. Rev. lat.-amer. Microbiol. 8, No. 3 (1967).

  4. Rhim, J. L., B. Simizu, andN. H. Wiebenga: Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. ges. Virusforsch.21, 243 (1967).

    Google Scholar 

  5. Reed, L. J., andH. A. Muench: A simple method of estimating 50% end points. Amer. J. Hyg.27, 493 (1938).

    Google Scholar 

  6. Fleck, A., andH. N. Munro: The precision of ultraviolet-absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim. biophys. Acta (Amst.)55, 571 (1962).

    Google Scholar 

  7. Salzman, N. P.: The rate of formation of vaccinia deoxyribonucleic acid and vaccinia virus. Virology10, 150 (1960).

    Google Scholar 

  8. Hamparian, V. V., M. R. Hilleman, andA. Kettler: Contributions to characterization and classification of animal viruses. Proc. Soc. exp. Biol. (N.Y.)112, 1040 (1963).

    Google Scholar 

  9. Stollar, V., T. M. Stevens, andR. W. Schlesinger: Studies on the nature of Dengue viruses. II. Characterization of viral RNA and effects of inhibitors of RNA synthesis. Virology30, 303 (1966).

    Google Scholar 

  10. David-West, T. S., andH. H. Labzoffsky: Studies on the site of replication of vesicular stomatitis virus. Arch. ges. Virusforsch.24, 30 (1968).

    Google Scholar 

  11. Barky, R. D.: The effects of actinomycin D and ultraviolet irradiation on the production of fowl plague virus. Virology24, 563 (1964).

    Google Scholar 

  12. Barry, R. D., D. R. Ives, andJ. G. Cruickshank: Participation of deoxyribonucleic acid in the multiplication of Influenza virus. Nature (Lond.)194, 1139 (1962).

    Google Scholar 

  13. Rubin, H., M. Baluda, andJ. E. Hotchin: The maturation of Western equine encephalomyelitis virus and its release from chick embryo cells in suspension. J. exp. Med.101, 205 (1955).

    Google Scholar 

  14. Taylor, J.: Inhibition of interferon action by actinomycin. Biochem. biophys. Res. Commun.14, 447 (1964).

    Google Scholar 

  15. Heller, E.: Enhancement of Chikungunya virus replication and inhibition of interferon production by actinomycin D. Virology21, 652 (1963).

    Google Scholar 

  16. Wong, K. T., S. Baron, H. B. Levy, andT. G. Ward: D actinomycin: Relative resistance of Green monkey kidney cell cultures to its action (32014). Proc. Soc. exp. Biol. (N.Y.)125, 65 (1967).

    Google Scholar 

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Author notes

  1. Celia E. Coto (Member of the Research Career of the “Consejo Nacional de Investigaciones Cientifícas y Técnicas de la Rca. Argentina”)

    Present address: Facultad de Medicina, Cátedra de Microbiología y Parasitología, Buenos Aires, Argentina

Authors and Affiliations

  1. Facultad de Medicina, Cátedra de Microbiología y Parasitología, Buenos Aires, Argentina

    Marieta D. de Vombergar

Authors
  1. Celia E. Coto
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  2. Marieta D. de Vombergar
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Additional information

This research was supported by a grant No. 2824 from the “Consejo Nacional de Investigaciones Científicas y Técnicas de la Rca. Argentina” and by the “Comision de Lucha contra la Fiebre Hemorrágica Argentina del Ministerio de Asistencia Social y Salud Pública de la Rca. Argentina”.

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Coto, C.E., de Vombergar, M.D. The effect of 5-iododeoxyuridine and actinomycin D on the multiplication of junin virus. Archiv f Virusforschung 27, 307–316 (1969). https://doi.org/10.1007/BF01249653

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  • DOI: https://doi.org/10.1007/BF01249653

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